Human Mammary Tumor Virus, Human Papilloma Virus, and Epstein-Barr Virus Infection Are Associated With Sporadic Breast Cancer Metastasis.

IF 1.8 Q3 ONCOLOGY Breast Cancer : Basic and Clinical Research Pub Date : 2020-11-24 eCollection Date: 2020-01-01 DOI:10.1177/1178223420976388
Mohammad Al Hamad, Ismail Matalka, Mazhar Salim Al Zoubi, Ivana Armogida, Rawan Khasawneh, Maysa Al-Husaini, Maher Sughayer, Saied Jaradat, Amjad D Al-Nasser, Chiara Maria Mazzanti
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引用次数: 16

Abstract

Background: Viral cause of sporadic breast cancer (SBC) has been suggested based on the experimental murine model of mammary tumor caused by mouse mammary tumor virus (MMTV), Epstein-Barr virus (EBV), and human papillomavirus (HPV). While some studies have demonstrated the presence of viral sequences of MMTV, HPV, and EBV in breast cancer cells, others failed. These contradictions may be attributed to the geographical distribution of breast cancer incidence and/or technical variations. In the current study, we aimed to investigate the correlation of MMTV, HPV, and EBV infections with the development of breast cancer in Jordanian patients.

Methods: One hundred SBC tissue samples were subjected to laser capture microdissection for the selection of tumor cells populations. Fluorescence polymerase chain reaction (PCR) was used to detect the presence of the MMTV env-like sequences. Real-time PCR was used for HPV and EBV detection, and EBV was further confirmed by chromogen in situ hybridization (CISH).

Results: Mouse mammary tumor virus, HPV, and EBV were detected in SBC in 11%, 21%, and 23%, respectively. Only 3 of 52 (5.7%) positive cases demonstrated multiple virus infections. However, 49 of 52 (94%) of the positive cases revealed the presence of 1 type of viral sequences. Consequently, 52% of the studied breast cancer cases were infected with at least 1 type of the aforementioned viruses.

Conclusions: The current cohort suggests that MMTV, HPV, and EBV have a potential role in the development of breast cancer and adding more reasons to proceed with the quest of a possible viral origin of breast cancer.

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人乳腺肿瘤病毒、人乳头瘤病毒和eb病毒感染与散发性乳腺癌转移有关
背景:基于小鼠乳腺肿瘤病毒(MMTV)、eb病毒(EBV)和人乳头瘤病毒(HPV)引起的小鼠乳腺肿瘤实验模型,提出了散发性乳腺癌(SBC)的病毒病因。虽然一些研究已经证明乳腺癌细胞中存在MMTV、HPV和EBV的病毒序列,但其他研究却失败了。这些矛盾可能归因于乳腺癌发病率的地理分布和/或技术差异。在目前的研究中,我们的目的是研究MMTV、HPV和EBV感染与约旦患者乳腺癌发展的相关性。方法:对100例SBC组织标本进行激光捕获显微解剖,筛选肿瘤细胞群。采用荧光聚合酶链反应(PCR)检测MMTV env样序列的存在。HPV和EBV检测采用实时荧光定量PCR, EBV检测采用染色体原位杂交(CISH)。结果:小鼠乳腺肿瘤病毒、HPV和EBV在SBC中的检出率分别为11%、21%和23%。52例阳性病例中仅有3例(5.7%)出现多重病毒感染。然而,52例阳性病例中有49例(94%)显示存在1型病毒序列。因此,在所研究的乳腺癌病例中,有52%至少感染了上述一种病毒。结论:目前的队列研究表明,MMTV、HPV和EBV在乳腺癌的发展中具有潜在的作用,这为继续探索乳腺癌可能的病毒起源提供了更多的理由。
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来源期刊
CiteScore
5.10
自引率
3.40%
发文量
22
审稿时长
8 weeks
期刊介绍: Breast Cancer: Basic and Clinical Research is an international, open access, peer-reviewed, journal which considers manuscripts on all areas of breast cancer research and treatment. We welcome original research, short notes, case studies and review articles related to breast cancer-related research. Specific areas of interest include, but are not limited to, breast cancer sub types, pathobiology, metastasis, genetics and epigenetics, mammary gland biology, breast cancer models, prevention, detection, therapy and clinical interventions, and epidemiology and population genetics.
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