An investigation into the impact of deleting one copy of the glutaredoxin-2 gene on diet-induced weight gain and the bioenergetics of muscle mitochondria in female mice fed a high fat diet.

IF 5.2 2区 生物学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY Redox Report Pub Date : 2020-12-01 DOI:10.1080/13510002.2020.1826750
Robert Gill, Sarah Mallay, Adrian Young, Ryan J Mailloux
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引用次数: 4

Abstract

Our group recently documented that male mice containing a deletion for one copy of the glutaredoxin-2 (Grx2) gene were completely protected from developing diet-induced obesity (DIO). Objectives: Here, we conducted a similar investigation but with female littermates. Results: In comparison to our recent publication using male mice, exposure of WT and GRX2+/- female mice to a HFD from 3-to-10 weeks of age did not induce any changes in body mass, circulating blood glucose, food intake, hepatic glycogen levels, or abdominal fat pad mass. Examination of the bioenergetics of muscle mitochondria revealed no changes in the rate of superoxide ( O 2 - )/hydrogen peroxide (H2O2) or O2 consumption under different states of respiration or alterations in lipid peroxidation adduct levels regardless of mouse strain or diet. Additionally, we measured the bioenergetics of mitochondria isolated from liver tissue and found that partial loss of GRX2 augmented respiration but did not alter ROS production. Discussion: Overall, our findings demonstrate there are sex differences in the protection of female GRX2+/- mice from DIO, fat accretion, intrahepatic lipid accumulation, and the bioenergetics of mitochondria from muscle and liver tissue.

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研究了在喂食高脂肪饮食的雌性小鼠中,删除一个glutaredoxin-2基因拷贝对饮食诱导的体重增加和肌肉线粒体生物能量学的影响。
我们的研究小组最近证明,含有一个glutaredoxin-2 (Grx2)基因拷贝缺失的雄性小鼠完全免受饮食性肥胖(DIO)的影响。目的:在这里,我们进行了类似的调查,但对象是雌性幼崽。结果:与我们最近发表的雄性小鼠相比,将WT和GRX2+/-雌性小鼠暴露于3至10周龄的HFD并没有引起体重、循环血糖、食物摄入量、肝糖原水平或腹部脂肪垫质量的任何变化。肌肉线粒体的生物能量学检查显示,在不同的呼吸状态下,超氧化物(O2∙-)/过氧化氢(H2O2)的速率或氧气消耗没有变化,脂质过氧化加合物水平也没有变化,而与小鼠品系或饮食无关。此外,我们测量了从肝组织分离的线粒体的生物能量学,发现GRX2的部分缺失增加了呼吸,但没有改变ROS的产生。讨论:总体而言,我们的研究结果表明,雌性GRX2+/-小鼠对DIO、脂肪积累、肝内脂质积累以及肌肉和肝组织线粒体生物能量的保护存在性别差异。
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来源期刊
Redox Report
Redox Report 生物-生化与分子生物学
CiteScore
6.10
自引率
0.00%
发文量
28
审稿时长
>12 weeks
期刊介绍: Redox Report is a multidisciplinary peer-reviewed open access journal focusing on the role of free radicals, oxidative stress, activated oxygen, perioxidative and redox processes, primarily in the human environment and human pathology. Relevant papers on the animal and plant environment, biology and pathology will also be included. While emphasis is placed upon methodological and intellectual advances underpinned by new data, the journal offers scope for review, hypotheses, critiques and other forms of discussion.
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