{"title":"Nucleotide sequencing of the HoxA gene cluster using Gorilla fosmid clones.","authors":"Takashi Kitano, Choong-Gon Kim, Naruya Saitou","doi":"10.7150/jgen.50468","DOIUrl":null,"url":null,"abstract":"<p><p>We sequenced the western gorilla (<i>Gorilla gorilla</i>) HoxA cluster region using seven fosmid clones, and found that the total tiling path sequence was 214,185 bp from the 5' non-genic region of HoxA1 to the 3' non-genic region of Evx1. We compared the nucleotide sequence with the gorilla genome sequence in the NCBI database, and the overall proportion of nucleotide difference was estimated to be 0.0005-0.0007. These estimates are lower than overall genomic polymorphism in gorillas.</p>","PeriodicalId":15834,"journal":{"name":"Journal of Genomics","volume":"8 ","pages":"80-83"},"PeriodicalIF":0.0000,"publicationDate":"2020-08-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.7150/jgen.50468","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Genomics","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.7150/jgen.50468","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2020/1/1 0:00:00","PubModel":"eCollection","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0
Abstract
We sequenced the western gorilla (Gorilla gorilla) HoxA cluster region using seven fosmid clones, and found that the total tiling path sequence was 214,185 bp from the 5' non-genic region of HoxA1 to the 3' non-genic region of Evx1. We compared the nucleotide sequence with the gorilla genome sequence in the NCBI database, and the overall proportion of nucleotide difference was estimated to be 0.0005-0.0007. These estimates are lower than overall genomic polymorphism in gorillas.
期刊介绍：
Journal of Genomics publishes papers of high quality in all areas of gene, genetics, genomics, proteomics, metabolomics, DNA/RNA, computational biology, bioinformatics, and other relevant areas of research and application. Articles published by the journal are rigorously peer-reviewed. Types of articles include: Research paper, Short research communication, Review or mini-reviews, Commentary, Database, Software.
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