Clonal dissemination of antibiotic resistance among Tunisian Mycoplasma gallisepticum isolates as revealed by gene-targeted sequencing analysis.

IF 1.3 4区农林科学 Q2 VETERINARY SCIENCES Avian Diseases Pub Date : 2020-10-15 DOI:10.1637/aviandiseases-D-20-00080R1

Imen Chniba, Safa Boujemaa, Boutheina Ben Abdelmoumen Mardassi, Boutheina Ben Abdelmoumen Mardassi

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Abstract

SummaryTo date, very little is known about avian mycoplasma infections in Tunisia. Mycoplasma gallisepticum is one of the most economically significant pathogen for poultry in Tunisia and worldwide. Based on the paucity of data regarding the genetic profiles and antibacterial behavior of M. gallisepticum strains in Tunisia, the present study was conducted. Genetic typing and phylogenetic relationships of 40 M. gallisepticum strains (20 Tunisian isolates, 19 international strains collection, and S6 reference strain) were investigated by gene-targeted sequencing (GTS) using 4 loci ( pvpA , mgc2 , vlhA and the InterGenic Spacer Region (IGSR) between the 16S and the 23S rRNA genes). GTS reveals 12 STs that were found to spread over 2 clonal complexes (CC) and 5 singletons.Emergence of enrofloxacin and spiramycin resistance among M. gallisepticum local isolates have been revealed using the broth microdilution method. Causal mutations have been identified by sequencing the quinolone-resistance determining region (QRDR) and domain II and V of 23S rRNA as well as the rplD and rplV genes for enrofloxacine- and macrolide-resistant isolates, respectively. The emersion of antibiotic resistance to enrofloxacin and spiramycin has been identified as being related to a distinctive clonal complex formed by 4 different STs (ST2, ST3, ST4 and ST5) which would suggest that this phenotype was clonally disseminated.

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通过基因靶向测序分析揭示突尼斯胆囊支原体分离株中抗生素耐药性的克隆传播。

摘要迄今为止，人们对突尼斯的禽支原体感染知之甚少。五倍子支原体是突尼斯乃至全球家禽中最具经济意义的病原体之一。由于突尼斯有关五倍子支原体菌株的遗传特征和抗菌行为的数据很少，因此开展了本研究。通过基因靶向测序（GTS），使用 4 个基因位点（pvpA、mgc2、vlhA 和 16S 与 23S rRNA 基因之间的基因间距区（IGSR））对 40 株 M. gallisepticum 菌株（20 株突尼斯分离株、19 株国际菌株集和 S6 参考菌株）的基因分型和系统发育关系进行了研究。使用肉汤微稀释法发现了当地分离的加里西普氏霉菌对恩诺沙星和螺旋霉素的耐药性。通过对恩诺沙星和大环内酯耐药分离株的喹诺酮耐药决定区（QRDR）、23S rRNA的II和V结构域以及rplD和rplV基因进行测序，确定了致病突变。恩诺沙星和螺旋霉素抗药性的出现已被确认与 4 个不同 ST（ST2、ST3、ST4 和 ST5）形成的独特克隆复合体有关，这表明这种表型是克隆传播的。

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来源期刊

Avian Diseases 农林科学-兽医学

CiteScore

2.40

自引率

7.10%

发文量

审稿时长

3 months

期刊介绍： Avian Diseases is an international journal dedicated to publishing original basic or clinical research of the highest quality from various disciplines including microbiology, immunology, pathology and epidemiology. Papers on avian diseases relevant to etiology, pathogenesis, diagnosis, treatment, and control are accepted. Manuscripts dealing with avian species other than poultry will be considered only if the subject is relevant to poultry health.

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