Proteins and Amino Acids Treated with Atmospheric Plasma Show Significantly Increased Bioavailability in Humans.

IF 2.3 Q3 NUTRITION & DIETETICS Nutrition and Metabolic Insights Pub Date : 2020-09-28 eCollection Date: 2020-01-01 DOI:10.1177/1178638820949239

Matthew H Sharp, Matthew W Stefan, Raad H Gheith, Dallen D Reber, Charlie R Ottinger, Jacob M Wilson, Ryan P Lowery

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Abstract

Background: Muscle mass is an important determinant of metabolic health and physical function. It has previously been demonstrated that the postprandial rise in circulating essential amino acids acts as the main stimulus for muscle protein synthesis (MPS). The current study investigated the postprandial plasma essential amino acid (EAA) and branched-chain amino acid (BCAA) responses of (1) Hydrolyzed whey protein isolate (HWPI) compared to plasma treated non-hydrolyzed whey protein isolate (PT-NHWPI), (2) standard branch-chain amino acids (S-BCAA) compared to plasma treated branch-chained amino acids (PT-BCAA), (3) standard pea protein (S-PP), compared to plasma treated pea protein (PT-PP), and (4) HWPI compared to PT-PP.

Methods: Ten subjects (24.6 ± 5.3 years; 178.8 ± 8.1 cm; 78.6 ± 10.1 kg) participated in a double-blind, randomized, crossover trial comparing four separate protein conditions (HWPI, PT-NHWPI, S-PP, PT-PP). A separate cohort of ten subjects (26.4 ± 7.4 years; 178.8 ± 5.9 cm; 85 ± 12.3 kg) participated in a double-blind randomized, crossover trial comparing two branch-chain amino acid conditions: S-BCAA and PT-BCAA. All conditions were administered following a 7-day washout. Plasma EAA and BCAA concentrations were assessed from blood donated by subjects at pre-consumption, 30-, 60-, 90-, 120-, and 180 minutes post-consumption.

Results: Blood plasma levels of total EAA and BCAA concentration were significantly greater in all treated conditions at 30-, 60-, 90-, and 120 minutes post consumption (P < .05). There were no differences between PT-PP and HWPI.

Discussion: All proteins significantly elevated EAAs, and BCAAs from basal levels. However, we conclude that the consumption of the treated proteins significantly raises blood levels of EAAs, and BCAAs to a greater extent across multiple dairy, vegan, and isolated BCAA conditions. Moreover, atmospheric plasma treatment of a vegan protein source makes its amino acid response similar to whey. Thus, protein supplementation with that has undergone Ingredient Optimized® atmospheric plasma treatment technology may be highly beneficial for improving the blood plasma amino acid response.

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经大气等离子体处理的蛋白质和氨基酸在人体内的生物利用度显著提高。

背景:肌肉质量是代谢健康和身体机能的重要决定因素。先前已经证明，餐后循环中必需氨基酸的增加是肌肉蛋白质合成(MPS)的主要刺激因素。目前的研究调查了(1)水解分离乳清蛋白(HWPI)与血浆处理的非水解分离乳清蛋白(PT-NHWPI)的餐后血浆必需氨基酸(EAA)和支链氨基酸(BCAA)的反应，(2)标准支链氨基酸(S-BCAA)与血浆处理的支链氨基酸(PT-BCAA)的比较，(3)标准豌豆蛋白(S-PP)与血浆处理的豌豆蛋白(PT-PP)的比较，(4)HWPI与PT-PP的比较。方法:10例(24.6±5.3岁);178.8±8.1 cm;78.6±10.1 kg)参加了一项双盲、随机、交叉试验，比较了四种不同的蛋白质状况(HWPI、PT-NHWPI、S-PP、PT-PP)。一个单独的队列，10名受试者(26.4±7.4岁;178.8±5.9 cm;85±12.3 kg)参加了一项双盲随机交叉试验，比较了S-BCAA和PT-BCAA两种支链氨基酸条件。所有条件均在7天的洗脱期后进行。在消耗前、消耗后30分钟、60分钟、90分钟、120分钟和180分钟对受试者献血的血浆EAA和BCAA浓度进行评估。结果:在摄入后30分钟、60分钟、90分钟和120分钟，所有治疗条件下的血浆总EAA和BCAA浓度水平均显著升高(P讨论:所有蛋白质均显著升高EAA和BCAAs，高于基础水平。然而，我们得出结论，在多种乳制品、素食和分离的BCAA条件下，摄入处理过的蛋白质显著提高了血液中EAAs和BCAAs的水平。此外，大气等离子体处理纯素蛋白源使其氨基酸反应类似于乳清。因此，补充经过Ingredient Optimized®大气血浆处理技术的蛋白质可能对改善血浆氨基酸反应非常有益。

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来源期刊

Nutrition and Metabolic Insights NUTRITION & DIETETICS-

CiteScore

3.30

自引率

0.00%

发文量

审稿时长

8 weeks

期刊介绍： Nutrition and Metabolic Insights is a peer-reviewed, open-access online journal focusing on all aspects of nutrition and metabolism. This encompasses nutrition, including the biochemistry of metabolism, exercise and associated physical processes and also includes clinical articles that relate to metabolism, such as obesity, lipidemias and diabetes. It includes research at the molecular, cellular and organismal levels. This journal welcomes new manuscripts for peer review on the following topics: Nutrition, including the biochemistry of metabolism, Exercise and associated physical processes, Clinical articles that relate to metabolism, such as obesity, lipidemias and diabetes, Research at the molecular, cellular and organismal levels, Other areas of interest include gene-nutrient interactions, the effects of hormones, models of metabolic function, macronutrient interactions, outcomes of changes in diet, and pathophysiology.