Havachoobe (Onosma dichroanthum Boiss) Root Extract Decreases the Hepatitis B Virus Surface Antigen Secretion in the PLC/PRF/5 Cell Line.

IF 4.3 3区材料科学 Q1 ENGINEERING, ELECTRICAL & ELECTRONIC ACS Applied Electronic Materials Pub Date : 2020-12-15 DOI:10.1159/000512140

Alireza Mohebbi, Fahimeh Azadi, Mohammad Mostakhdem Hashemi, Fatemeh Sana Askari, Nazanin Razzaghi

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Abstract

Background: Many efforts are currently focused on functional treatment of the hepatitis B virus (HBV). This can be done by suppressing the secretion of HBV surface antigen (HBsAg). Scientific communities are very interested in natural products in that respect.

Objective: Use of root extract of Havachoobe (Onosma dichroanthum BoissI), a Northern Iranian native medical herb, for assessment of its anti-HBsAg secretion activity.

Methods: Havachoobe had been bought at a nearby apothecary store. Plant root extract was obtained using a hydroalcoholic process. Cytotoxic activity of the extract was examined on PLC/PRF/5 cells using MTT assay. ELISA has been used to measure HBsAg in the treated cell line supernatants. In addition, real-time PCR analysis was performed to evaluate the expression of HBsAg before and after treatment of Onosma in vitro.

Results: The results showed very low root extract cytotoxicity at concentrations under 8 μg/mL. Tissue culture infectious dose 50 was obtained at 63.78 μg/mL. In a dose-dependent and time-dependent manner, a significantly reduced HBsAg secretion was observed at a concentration of 8 ppm at 12 h post-treatment. The real-time PCR result showed relative decreased HBsAg expression at all doses at 12 h post-treatment time.

Discussion: In this study, we first reported anti-HBsAg activity on an Iranian herbal medicine. Havachoobe root extract was shown to be able to inhibit HBsAg in a dose-dependent and time-dependent manner. We find the extract exerts its inhibitory effect of HBsAg by targeting transcription of HBsAg.

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Havachoobe（Onosma dichroanthum Boiss）根提取物可减少 PLC/PRF/5 细胞系中乙型肝炎病毒表面抗原的分泌。

背景：目前，许多工作都集中在乙型肝炎病毒（HBV）的功能性治疗上。这可以通过抑制 HBV 表面抗原（HBsAg）的分泌来实现。科学界对这方面的天然产品非常感兴趣：使用 Havachoobe（Onosma dichroanthum BoissI）的根提取物评估其抗 HBsAg 分泌的活性。方法：Havachoobe 是在附近的药店购买的，植物根提取物是用水醇法提取的。使用 MTT 法检测提取物对 PLC/PRF/5 细胞的细胞毒活性。酶联免疫吸附法（ELISA）用于检测处理过的细胞系上清液中的 HBsAg。此外，还进行了实时 PCR 分析，以评估体外处理 Onosma 前后 HBsAg 的表达情况：结果表明，当根提取物的浓度低于 8 μg/mL 时，其细胞毒性非常低。组织培养感染剂量 50 为 63.78 μg/mL。在剂量依赖性和时间依赖性的作用下，浓度为 8 ppm 时，处理后 12 小时的 HBsAg 分泌明显减少。实时聚合酶链式反应（real-time PCR）结果显示，在处理后 12 小时，所有剂量的 HBsAg 表达量都相对减少：本研究首次报道了一种伊朗草药的抗 HBsAg 活性。研究表明，Havachoobe 根提取物能够以剂量依赖性和时间依赖性的方式抑制 HBsAg。我们发现该提取物通过靶向 HBsAg 的转录来发挥抑制 HBsAg 的作用。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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