Effect of stem cells-based therapy on astrogliosis in stroke subjected-mice.

Q1 Biochemistry, Genetics and Molecular Biology Stem cell investigation Pub Date : 2020-12-15 eCollection Date: 2020-01-01 DOI:10.21037/sci-2020-031

Kobra Akhoundzadeh, Abedin Vakili

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引用次数: 1

Abstract

This study was planned to continue our previous study to assess effect of combination therapy bone marrow stromal cells (BMSCs) with exercise (EX) and triiodothyronine (T₃) on stroke-induced astrogliosis in mice. Stroke subjected-mice were divided into five monotherapy groups including sham, control, BMSCs, EX and T₃; and three combination therapy groups including BMSCs + EX, BMSCs + T₃ and BMSCs + EX + T₃. Astrogliosis was assessed in ipsilateral hemisphere at day 7 after MCAO. Combination therapy BMSCs with EX and T₃ could significantly decrease stroke-induced astrogliosis. However, monotherapy with BMSCs or EX also improved changes of glial fibrillary acidic protein (GFAP)-positive cells following stroke. Combination therapy BMSCs with EX and T₃ didn't have any added effect on astrogliosis compared to monotherapy with BMSCs or EX. With comparing the present findings with the results of neurobehavioral functioning in our earlier study, it seems that decrease of astrogliosis could be helpful for stroke recovery.

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干细胞治疗对脑卒中小鼠星形胶质细胞形成的影响。

本研究计划继续我们先前的研究，以评估骨髓基质细胞(BMSCs)与运动(EX)和三碘甲状腺原氨酸(T3)联合治疗小鼠脑卒中诱导的星形胶质细胞形成的影响。将脑卒中小鼠分为假手术组、对照组、骨髓间充质干细胞组、EX组和T3组;BMSCs + EX、BMSCs + T3、BMSCs + EX + T3三个联合治疗组。MCAO术后第7天对同侧半球星形胶质细胞形成进行评估。BMSCs与EX和T3联合治疗可显著减少脑卒中诱导的星形胶质细胞增生。然而，骨髓间充质干细胞或EX单药治疗也能改善脑卒中后胶质纤维酸性蛋白(GFAP)阳性细胞的变化。与BMSCs或EX单药治疗相比，BMSCs与EX和T3联合治疗对星形胶质细胞增生没有任何额外的作用。将本研究结果与我们早期研究的神经行为功能结果进行比较，似乎星形胶质细胞增生的减少可能有助于中风的恢复。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Stem cell investigation Biochemistry, Genetics and Molecular Biology-Developmental Biology

CiteScore

5.80

自引率

0.00%

发文量

期刊介绍： The Stem Cell Investigation (SCI; Stem Cell Investig; Online ISSN: 2313-0792) is a free access, peer-reviewed online journal covering basic, translational, and clinical research on all aspects of stem cells. It publishes original research articles and reviews on embryonic stem cells, induced pluripotent stem cells, adult tissue-specific stem/progenitor cells, cancer stem like cells, stem cell niche, stem cell technology, stem cell based drug discovery, and regenerative medicine. Stem Cell Investigation is indexed in PubMed/PMC since April, 2016.