Activation of RAW264.7 cells by PCp-I, a polysaccharide from Psoralea corylifolia L, through NF-κB/MAPK signalling pathway.

Honglin Wang, Xiaoqing Xu, Zhenhua Yin, Mengke Wang, Baoguang Wang, Changyang Ma, Jinmei Wang, Wenyi Kang
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引用次数: 8

Abstract

PCp-I is a polysaccharide isolated and identified from the Psoralea corylifolia L. by our research group. In this study, the immunomodulatory effects of PCp-I on RAW264.7 cells was evaluated. PCp-I could enhance the level of NO along with up-regulation of iNOS mRNA in RAW264.7 cells. The PCp-I could significantly up-regulate the mRNA expression of TNF-α and IL-6 in RAW264.7 cells, and then the expression of TNF-α, IL-6, ROS and the phagocytic activity were increased. Additionally, PCp-I could significantly up-regulate the phosphorylation level of p65, p38, ERK and JNK proteins, which proved that PCp-I could activate the macrophages by MAPKs and NF-κB signalling pathway and the TLR4 may be one of the receptors of PCp-I regulate the RAW264.7 cells.

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补骨脂多糖pcp - 1通过NF-κB/MAPK信号通路激活RAW264.7细胞。
pcp - 1是课题组从补骨脂中分离鉴定的多糖。本研究评估了pcp - 1对RAW264.7细胞的免疫调节作用。pcp - 1能提高RAW264.7细胞NO水平,上调iNOS mRNA表达。pcp - 1可显著上调RAW264.7细胞中TNF-α、IL-6 mRNA的表达,进而提高TNF-α、IL-6、ROS的表达及吞噬活性。此外,pcp - 1可以显著上调p65、p38、ERK和JNK蛋白的磷酸化水平,证明pcp - 1可以通过MAPKs和NF-κB信号通路激活巨噬细胞,TLR4可能是pcp - 1调节RAW264.7细胞的受体之一。
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来源期刊
CiteScore
4.00
自引率
0.00%
发文量
88
审稿时长
15 weeks
期刊介绍: International Journal of Immunopathology and Pharmacology is an Open Access peer-reviewed journal publishing original papers describing research in the fields of immunology, pathology and pharmacology. The intention is that the journal should reflect both the experimental and clinical aspects of immunology as well as advances in the understanding of the pathology and pharmacology of the immune system.
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