LINC00641 Inhibits the Development of Cutaneous Squamous Cell Carcinoma By Downregulating miR-424 in A431 Cells.

IF 2.4 4区医学 Q3 MEDICINE, RESEARCH & EXPERIMENTAL Cancer Biotherapy and Radiopharmaceuticals Pub Date : 2024-09-01 Epub Date: 2021-04-20 DOI:10.1089/cbr.2020.4325

Wenmin Liu, Xinxin Liu

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Abstract

Background: Cutaneous squamous cell carcinoma (CSCC) is the most deadly disease among nonmelanoma skin cancers. LINC00641 plays a role in various cancers, but its role in CSCC has not been reported so far. Methods and Materials: The expression of LINC00641 and miR-424 in cells was detected by RT-qPCR. CCK-8 and colony formation assay were used to detect the proliferation of cells. Western blot was used to detect the expression levels of proliferation-, invasion-, and migration-related proteins. Wound Healing and Transwell experiments detected the ability of cell invasion and migration. In animal experiments, a tumor-bearing model was established in nude mice, and tumor volume was measured and photographed. The expression levels of proliferation-, invasion-, and migration-related proteins were detected by Western blot. Results: The expression of LINC00641 was significantly decreased in CSCC cell lines. The overexpression of LINC00641 at the cellular level inhibited the proliferation and migration of CSCC cell line A431 by downregulating the expression of miR-424. The overexpression of LINC00641 in animals inhibited the tumor volume of nude mice by downregulating the expression of miR-424 to inhibit the expression of proliferation- and migration-related proteins. Conclusion: LINC00641 inhibits the development of CSCC by downregulating miR-424.

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LINC00641 通过下调 A431 细胞中的 miR-424 抑制皮肤鳞状细胞癌的发展

背景：皮肤鳞状细胞癌（CSCC）是非黑色素瘤皮肤癌中最致命的疾病。LINC00641 在多种癌症中发挥作用，但其在 CSCC 中的作用尚未见报道。方法和材料：通过 RT-qPCR 检测细胞中 LINC00641 和 miR-424 的表达。用 CCK-8 和集落形成试验检测细胞的增殖情况。Western 印迹法检测增殖、侵袭和迁移相关蛋白的表达水平。伤口愈合和 Transwell 实验检测细胞的侵袭和迁移能力。在动物实验中，建立了裸鼠肿瘤模型，并对肿瘤体积进行了测量和拍照。用 Western 印迹法检测增殖、侵袭和迁移相关蛋白的表达水平。结果显示LINC00641在CSCC细胞系中的表达明显下降。在细胞水平过表达 LINC00641 可通过下调 miR-424 的表达抑制 CSCC 细胞株 A431 的增殖和迁移。在动物体内过表达 LINC00641 可通过下调 miR-424 的表达来抑制增殖和迁移相关蛋白的表达，从而抑制裸鼠的肿瘤体积。结论LINC00641 通过下调 miR-424 抑制 CSCC 的发展。

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来源期刊

Cancer Biotherapy and Radiopharmaceuticals 医学-核医学

CiteScore

7.80

自引率

2.90%

发文量

审稿时长

3 months

期刊介绍： Cancer Biotherapy and Radiopharmaceuticals is the established peer-reviewed journal, with over 25 years of cutting-edge content on innovative therapeutic investigations to ultimately improve cancer management. It is the only journal with the specific focus of cancer biotherapy and is inclusive of monoclonal antibodies, cytokine therapy, cancer gene therapy, cell-based therapies, and other forms of immunotherapies. The Journal includes extensive reporting on advancements in radioimmunotherapy, and the use of radiopharmaceuticals and radiolabeled peptides for the development of new cancer treatments.