Redox imbalance in Crohn's disease patients is modulated by Azathioprine.

IF 5.2 2区生物学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY Redox Report Pub Date : 2021-12-01 DOI:10.1080/13510002.2021.1915665

Mohammad Javad Tavassolifar, Mostafa Changaei, Zahra Salehi, Fatemeh Ghasemi, Moslem Javidan, Mohammad Hossein Nicknam, Mohammad Reza Pourmand

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引用次数: 4

Abstract

Background: Crohn's disease (CD) is a chronic inflammatory disease without a specific cause. Inflammation in these patients can disturb the oxidants/antioxidants balance and results in oxidative stress that plays a destructive role. This study aimed to evaluate the gene expression of sod1, sod2, cat, nrf2 and gp91phox in CD patients before and after Azathioprine (Aza) consumption.

Method: Peripheral bloodmononuclear cells (PBMCs) were separated from CD patients (n= 15, mean age = 33.6 ± 1.8) before and after treatment with Aza and healthy controls (n= 15, mean age = 31.5 ± 1.2). The expression levels of sod1, sod2, cat, nrf2 and gp91phox were measured in byusing real-time qRT-PCR technique.

Result: The expression levels of gp91phox (P-value < 0.001), cat (P-value < 0.05), sod1 (P-value < 0.001), nrf2 (P-value < 0.001) were significantly increased compared to control group. Following treatment with Aza, the decreased expression levels of gp91phox (P-value < 0.05), cat (P-value < 0.05), sod1(P-value < 0.001) and nrf2 (P-value < 0.001) were observed in CD patients.

Conclusion: Overall, our results showed that prescription of Azathioprine can lead to the altered expression of redox system-related genes in patients with CD.

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硫唑嘌呤可调节克罗恩病患者的氧化还原失衡。

背景:克罗恩病(CD)是一种慢性炎症性疾病，病因不明。这些患者的炎症会扰乱氧化剂/抗氧化剂的平衡，导致氧化应激，起破坏性作用。本研究旨在评估硫唑嘌呤(Azathioprine, Aza)摄入前后CD患者体内sod1、sod2、cat、nrf2和gp91phox基因的表达情况。方法:分别从CD患者(n= 15，平均年龄= 33.6±1.8)和健康对照组(n= 15，平均年龄= 31.5±1.2)中分离外周血单个核细胞(PBMCs)。采用实时荧光定量pcr技术检测sod1、sod2、cat、nrf2和gp91phox的表达水平。结果:gp91phox (P-value cat) (P-value sod1(P-value nrf2) (P-value gp91phox (P-value cat) (P-value sod1(P-value nrf2 (P-value))的表达水平。结论:总体而言，我们的研究结果表明，硫唑嘌呤处方可导致CD患者氧化还原系统相关基因的表达改变。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Redox Report 生物-生化与分子生物学

CiteScore

6.10

自引率

0.00%

发文量

审稿时长

>12 weeks

期刊介绍： Redox Report is a multidisciplinary peer-reviewed open access journal focusing on the role of free radicals, oxidative stress, activated oxygen, perioxidative and redox processes, primarily in the human environment and human pathology. Relevant papers on the animal and plant environment, biology and pathology will also be included. While emphasis is placed upon methodological and intellectual advances underpinned by new data, the journal offers scope for review, hypotheses, critiques and other forms of discussion.