Gut Microbiota Diversity in HIV-Infected Patients on Successful Antiretroviral Treatment is Linked to Sexual Preferences but not CD4 Nadir

IF 2.9 4区 医学 Q3 IMMUNOLOGY Archivum Immunologiae et Therapiae Experimentalis Pub Date : 2021-05-13 DOI:10.1007/s00005-021-00616-7
Elżbieta Jabłonowska, Joanna Strzelczyk, Anna Piekarska, Kamila Wójcik-Cichy
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引用次数: 2

Abstract

The effects of HIV infection and antiretroviral therapy (ART) on the gut microbiome are poorly understood and the literature data are inconsistent. The aim of this study was to assess the alpha and beta diversity of the fecal microbiota in HIV-infected patients on successful antiretroviral therapy with regard to sexual preferences and CD4 nadir. Thirty-six HIV-infected ART-treated patients with HIV viremia below 20 copies/ml and CD4 > 500 cells/μl were divided into two subgroups based on CD4 nadir. The composition of the intestinal microbiota was assessed by 16SrRNA sequencing (MiSeq Illumina). The alpha and beta diversity were analyzed according to CD4 nadir count and sexual preference. Several alpha diversity indexes were significantly higher in the MSM group than in heterosexual patients. The alpha diversity did not differ significantly between patients with CD4 nadir > 500 cells/μl and CD4 nadir < 200 cells/μl. Beta diversity was also associated with sexual preference. A significant difference in Weighted Unifrac was observed between all MSM and all non-MSM participants (p = 0.001). The MSM group was more diverse and demonstrated greater distances in Weighted Unifrac than the non-MSM group. The relative abundance of the Prevotella enterotype was higher in the MSM than the non-MSM group. Sexual preferences demonstrated a stronger influence on alpha and beta diversity in HIV-infected patients following successful antiretroviral treatment than HIV infection itself. The observed lack of association between CD4 nadir and alpha and beta diversity may be caused by the restoration of the faecal microbiota following antiretroviral treatment.

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成功接受抗逆转录病毒治疗的hiv感染者肠道微生物群多样性与性偏好有关,但与CD4最低点无关
HIV感染和抗逆转录病毒治疗(ART)对肠道微生物组的影响尚不清楚,文献数据也不一致。本研究的目的是评估成功接受抗逆转录病毒治疗的艾滋病毒感染者粪便微生物群的α和β多样性与性偏好和CD4最低点的关系。将36例HIV病毒血症低于20拷贝/ml、CD4 + gt; 500细胞/μl的HIV感染art治疗患者按CD4最低点分为2个亚组。通过16SrRNA测序(MiSeq Illumina)评估肠道微生物群的组成。根据CD4最低点计数和性取向分析α和β多样性。男男性行为组的几个α多样性指数明显高于异性恋患者。CD4最低点> 500 cells/μl和CD4最低点< 200 cells/μl患者的α多样性无显著差异。β多样性也与性偏好有关。在所有男男性行为者和所有非男男性行为者之间观察到加权Unifrac的显著差异(p = 0.001)。与非男同性恋者相比,男同性恋者群体在加权Unifrac中表现出更大的多样性和距离。在男男性行为者中,普雷沃氏菌的相对丰度高于非男男性行为者。在抗逆转录病毒治疗成功后,性偏好对艾滋病毒感染者α和β多样性的影响比艾滋病毒感染本身更大。观察到的CD4最低点与α和β多样性之间缺乏关联可能是由抗逆转录病毒治疗后粪便微生物群的恢复引起的。
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来源期刊
CiteScore
5.90
自引率
0.00%
发文量
26
审稿时长
>12 weeks
期刊介绍: Archivum Immunologiae et Therapiae Experimentalis (AITE), founded in 1953 by Ludwik Hirszfeld, is a bimonthly, multidisciplinary journal. It publishes reviews and full original papers dealing with immunology, experimental therapy, immunogenetics, transplantation, microbiology, immunochemistry and ethics in science.
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