In vitro plant regeneration system for date palm (Phoenix dactylifera L.): effect of chelated iron sources.

IF 3.6 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Journal, genetic engineering & biotechnology Pub Date : 2021-06-01 DOI:10.1186/s43141-021-00177-4
Ahmed Madi Waheed Al-Mayahi
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引用次数: 9

Abstract

Background: Iron chelate sources and their concentrations are important factors in in vitro propagation of date palm. This study's objective was to investigate the effect of the iron chelated form on the growth and development of tissue cultures of Barhee cultivar.

Results: The addition of FeEDDHA to the culture medium was more effective than FeEDTA on callus growth, shoot regeneration, and the number of shoots per jar, where the best result (220.8mg callus, 86.67% and 17.2 shoots per jar, respectively) was obtained by using 93.5 mg L-1 FeEDDHA (5.6 mg L-1 Fe), compared with other treatments. The results also indicate that using 93.5 mg L-1 FeEDDHA (5.6 mg L-1 Fe) as a supplement can decrease antioxidant enzymes CAT and POD activity compared to the rest of the treatments. Medium equipped with 187.0 mg L-1 FeEDDHA (11.2 mg L-1Fe) had the highest rooting percentage and number of roots per shoot than other treatments. The biochemical analysis results showed that treatments with FeEDDHA of 280.5 mg L-1 (16.8 mg L-1 Fe) and 187.0 mg L-1 (11.2 mg L-1Fe) significantly increased the iron content. The results showed that shoot maximum chlorophyll and endogenous IAA level content were recorded in a medium supplemented with 187.0 mg L-1 FeEDDHA (11.2 mg L-1Fe) as Fe source.

Conclusion: FeEDDHA used in the present study was proven to be a promising iron chelate source in comparison with the FeEDTA sources.

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枣椰树离体植株再生系统:螯合铁源的影响。
背景:铁螯合物来源及其浓度是影响枣椰树离体繁殖的重要因素。本研究的目的是研究铁螯合形式对巴喜品种组织培养物生长发育的影响。结果:在培养基中添加FeEDDHA对愈伤组织生长、新芽再生和每罐新芽数的影响均优于FeEDTA,其中添加93.5 mg L-1 FeEDDHA (5.6 mg L-1 Fe)处理的愈伤组织再生效果最佳,分别为220.8mg、86.67%和17.2芽/罐。结果还表明,与其他处理相比,添加93.5 mg L-1 FeEDDHA (5.6 mg L-1 Fe)可降低抗氧化酶CAT和POD活性。添加187.0 mg L-1 FeEDDHA (11.2 mg L-1 fe)的培养基生根率和单根数最高。生化分析结果表明,饲料dha添加量为280.5 mg L-1 (16.8 mg L-1Fe)和187.0 mg L-1 (11.2 mg L-1Fe)显著提高了铁含量。结果表明,在添加187.0 mg L-1 FeEDDHA (11.2 mg L-1Fe)为铁源的培养基中,茎部叶绿素含量和内源IAA含量最高。结论:与FeEDTA源相比,本研究中使用的FeEDDHA被证明是一种有前途的铁螯合源。
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