Comparative Efficacy of Two Types of Antibiotic Mixtures in Gut Flora Depletion in Female C57BL/6 Mice.

IF 1.3 4区 农林科学 Q2 VETERINARY SCIENCES Comparative medicine Pub Date : 2021-06-01 Epub Date: 2021-06-04 DOI:10.30802/AALAS-CM-21-000023
David R Goulding, Page H Myers, Angela B Dickerson, Molly M Comins, Rebecca A Wiltshire, Terry L Blankenship-Paris
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引用次数: 4

Abstract

Over the last decade, interest in the role of the microbiome in health and disease has increased. The use of germ-free animals and depletion of the microbial flora using antimicrobials are 2 methods commonly used to study the microbiome in laboratory mice. Germ-free mice are born, raised, and studied in isolators in the absence of any known microbes; however, the equipment, supplies, and training required for the use of these mice can be costly and time-consuming. The use of antibiotics to decrease the microbial flora does not require special equipment, can be used for any mouse strain, and is relatively inexpensive; however, mice treated in this manner still retain microbes and they do not live in a germ-free environment. One commonly used antibiotic cocktail regimen uses ampicillin, neomycin, metronidazole, and vancomycin in the drinking water for 2 to 4 wk. We found that the palatability of this mixture is low, resulting in weight loss and leading to removal of mice from the study. The addition of sucralose to the medicated water and making wet food (mash) with the medicated water improved intake; however, the low palatability still resulted in a high number of mice requiring removal. The current study evaluated a new combination of antibiotics designed to reduce the gut microbiota while maintaining body weights. C57BL/6NCrl mice were placed on one of the following drinking water regimens: ampicillin/neomycin/metronidazole/vancomycin water (n = 16), enrofloxacin/ampicillin water ( n = 12), or standard reverse osmosis deionized water (RODI) ( n = 11). During an 8 day regimen, mice were weighed and water consumption was measured. Feces were collected before and after 8 d of treatment. Quantitative real-time PCR (real-time qPCR) for 16S bacterial ribosome was performed on each sample, and values were compared among groups. The combination of enrofloxacin and ampicillin improved water intake, together with a greater reduction in gut flora.

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两种混合抗生素对雌性C57BL/6小鼠肠道菌群减少的比较疗效。
在过去的十年中,人们对微生物组在健康和疾病中的作用的兴趣有所增加。使用无菌动物和使用抗菌剂消耗微生物菌群是研究实验室小鼠微生物组的常用方法。无菌小鼠是在没有任何已知微生物的隔离环境中出生、饲养和研究的;然而,使用这些鼠标所需的设备、用品和培训既昂贵又耗时。使用抗生素来减少微生物菌群不需要特殊的设备,可以用于任何小鼠品系,而且相对便宜;然而,以这种方式处理的老鼠仍然保留着微生物,它们并不是生活在一个无菌的环境中。一种常用的抗生素鸡尾酒疗法是在饮用水中加入氨苄西林、新霉素、甲硝唑和万古霉素2至4周。我们发现这种混合物的适口性很低,导致体重减轻,并导致小鼠退出研究。在药水中加入三氯蔗糖,用药水制作湿食品(醪),提高了摄取量;然而,低适口性仍然导致大量老鼠需要移除。目前的研究评估了一种新的抗生素组合,旨在减少肠道微生物群,同时保持体重。C57BL/6NCrl小鼠分别饮用氨苄西林/新霉素/甲硝唑/万古霉素水(n = 16)、恩诺沙星/氨苄西林水(n = 12)或标准反渗透去离子水(n = 11)。在8天的疗程中,测量小鼠的体重和饮水量。分别于治疗前和治疗后8 d收集粪便。对每个样品进行16S细菌核糖体的实时荧光定量PCR (real-time qPCR),并进行组间数值比较。恩诺沙星和氨苄西林的联合使用改善了水的摄入量,同时肠道菌群的减少也更大。
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来源期刊
Comparative medicine
Comparative medicine 医学-动物学
CiteScore
1.90
自引率
0.00%
发文量
71
审稿时长
6-12 weeks
期刊介绍: Comparative Medicine (CM), an international journal of comparative and experimental medicine, is the leading English-language publication in the field and is ranked by the Science Citation Index in the upper third of all scientific journals. The mission of CM is to disseminate high-quality, peer-reviewed information that expands biomedical knowledge and promotes human and animal health through the study of laboratory animal disease, animal models of disease, and basic biologic mechanisms related to disease in people and animals.
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