{"title":"Gold Nanoparticles with Dexmedetomidine Regulate GSK-3<i>β</i> to Reduce Neurocognitive Effects in Anesthetized Rats.","authors":"Xiaoxia Zhang, Zumin Xing, Jiyuan Li, Shuyi Tang, Yiwen Zhang","doi":"10.1166/jnn.2021.18745","DOIUrl":null,"url":null,"abstract":"<p><p>The aim of this study was to explore the neurocognitive effects of dexmedetomidine-loaded gold nanoparticles (AuNPs-dexmedetomidine) on anesthetized rats. Sixty Sprague Dawley rats (age, 2-3 weeks; weight, 250-280 g) were randomly divided into three groups (<i>n</i> = 20): the control group and two groups that received intraperitoneal injection of AuNPs-dexmedetomidine at 50 and 100 <i>μ</i>g/kg each. Western blotting and RT-PCR were used to determine the protein and mRNA expression of GSK-3<i>β</i>, respectively. Compared with that in the control group, GSK-3<i>β</i> expression in AuNP-dexmedetomidine groups increased (P < 0.05). The protein expression of GSK-3<i>β</i> was higher and mRNA expression was significantly lower in the 100 <i>μ</i>g/kg AuNP-dexmedetomidine group (P < 0.05). AuNPs-dexmedetomidine reduced the neurocognitive effect on anesthetized rats through the regulation of the GSK-3<i>β</i> signaling pathway.</p>","PeriodicalId":16417,"journal":{"name":"Journal of nanoscience and nanotechnology","volume":null,"pages":null},"PeriodicalIF":0.0000,"publicationDate":"2021-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of nanoscience and nanotechnology","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1166/jnn.2021.18745","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0
Abstract
The aim of this study was to explore the neurocognitive effects of dexmedetomidine-loaded gold nanoparticles (AuNPs-dexmedetomidine) on anesthetized rats. Sixty Sprague Dawley rats (age, 2-3 weeks; weight, 250-280 g) were randomly divided into three groups (n = 20): the control group and two groups that received intraperitoneal injection of AuNPs-dexmedetomidine at 50 and 100 μg/kg each. Western blotting and RT-PCR were used to determine the protein and mRNA expression of GSK-3β, respectively. Compared with that in the control group, GSK-3β expression in AuNP-dexmedetomidine groups increased (P < 0.05). The protein expression of GSK-3β was higher and mRNA expression was significantly lower in the 100 μg/kg AuNP-dexmedetomidine group (P < 0.05). AuNPs-dexmedetomidine reduced the neurocognitive effect on anesthetized rats through the regulation of the GSK-3β signaling pathway.
期刊介绍：
JNN is a multidisciplinary peer-reviewed journal covering fundamental and applied research in all disciplines of science, engineering and medicine. JNN publishes all aspects of nanoscale science and technology dealing with materials synthesis, processing, nanofabrication, nanoprobes, spectroscopy, properties, biological systems, nanostructures, theory and computation, nanoelectronics, nano-optics, nano-mechanics, nanodevices, nanobiotechnology, nanomedicine, nanotoxicology.
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