Physiological and genomic analysis of newly-isolated polysaccharide synthesizing cyanobacterium Chroococcus sp. FPU101 and chemical analysis of the exopolysaccharide.

IF 0.8 4区 生物学 Q4 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Journal of General and Applied Microbiology Pub Date : 2021-11-25 Epub Date: 2021-07-10 DOI:10.2323/jgam.2021.02.002
Shinya Yoshikawa, Yu Kanesaki, Akira Uemura, Kazumasa Yamada, Maiko Okajima, Tatsuo Kaneko, Kaori Ohki
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引用次数: 2

Abstract

A unicellular cyanobacterium that produces a large amount of exopolysaccharide (EPS) was isolated. The isolate, named Chroococcus sp. FPU101, grew between 20 and 30°C and at light intensities between 10 and 80 μmol m-2 s-1. Purified EPS from Chroococcus sp. FPU101 had a molecular size of 5.9 × 103 kDa and contained galactose, rhamnose, fucose, xylose, mannose, glucose, galacturonic acid, and glucuronic acid at a molar ratio of 17.2:15.9:14.1:11.0:9.6:9.5:13.0:9.7. The EPS content significantly increased when the NaCl concentration in the medium was increased from 1.7 to 100 mM. However, high NaCl concentrations did not significantly affect the molecular size or chemical composition of the EPS. The genes wza, wzb, wzc, wzx, wzy, and wzz that are involved in EPS synthesis were conserved in the genome of Chroococcus sp. FPU101, which was sequenced in this study. These results suggest that the Wzy-dependent pathway is potentially involved in EPS production in this organism.

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蓝藻双球菌FPU101合成新分离多糖的生理和基因组分析及外多糖的化学分析。
分离出一种产生大量胞外多糖(EPS)的单细胞蓝藻。该菌株被命名为Chroococcus sp. FPU101,生长温度在20 ~ 30℃,光照强度在10 ~ 80 μmol m-2 s-1。从choococcus sp. FPU101纯化的EPS分子量为5.9 × 103 kDa,含有半乳糖、鼠李糖、焦糖、木糖、甘露糖、葡萄糖、半乳糖醛酸和葡萄糖醛酸,摩尔比为17.2:15.9:14.1:11.0:9.6:9.5:13.0:9.7。当培养基中NaCl浓度从1.7 mM增加到100 mM时,EPS含量显著增加,但高NaCl浓度对EPS的分子大小和化学组成没有显著影响。参与EPS合成的基因wza、wzb、wzc、wzx、wzy和wzz在本研究测序的绒球菌FPU101基因组中是保守的。这些结果表明,wzy依赖性途径可能参与了这种生物体中EPS的产生。
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来源期刊
Journal of General and Applied Microbiology
Journal of General and Applied Microbiology 生物-生物工程与应用微生物
CiteScore
2.40
自引率
0.00%
发文量
42
审稿时长
6-12 weeks
期刊介绍: JGAM is going to publish scientific reports containing novel and significant microbiological findings, which are mainly devoted to the following categories: Antibiotics and Secondary Metabolites; Biotechnology and Metabolic Engineering; Developmental Microbiology; Environmental Microbiology and Bioremediation; Enzymology; Eukaryotic Microbiology; Evolution and Phylogenetics; Genome Integrity and Plasticity; Microalgae and Photosynthesis; Microbiology for Food; Molecular Genetics; Physiology and Cell Surface; Synthetic and Systems Microbiology.
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