Altered Expression of miR-575 in Glioma is Related to Tumor Cell Proliferation, Migration, and Invasion.

IF 3.9 4区医学 Q2 NEUROSCIENCES NeuroMolecular Medicine Pub Date : 2022-06-01 Epub Date: 2021-07-16 DOI:10.1007/s12017-021-08679-7

Guangxin Wei, Shengjun Li, Pengcheng Wang, Shouxian Wang, Yujing Zhao

{"title":"Altered Expression of miR-575 in Glioma is Related to Tumor Cell Proliferation, Migration, and Invasion.","authors":"Guangxin Wei, Shengjun Li, Pengcheng Wang, Shouxian Wang, Yujing Zhao","doi":"10.1007/s12017-021-08679-7","DOIUrl":null,"url":null,"abstract":"<p><p>Glioma is a kind of brain tumor with low overall survival and treatment success rates in the advanced stage. Evidence has shown microRNA-575 (miR-575) plays an important role in the generation and development of various cancers. This study aimed to explore the function of miR-575 in the prognosis and cell biological behavior of glioma. qRT-PCR was used to evaluate the expression of miR-575 in glioma tissues and cells, Kaplan-Meier survival analysis and Cox regression analysis were used to evaluate the prognostic value. The proliferation ability of glioma cells was determined by MTT assay; the invasion and migration abilities were determined by transwell assays. Compared with normal brain tissues, the expression of miR-575 in glioma tissue cells was significantly up-regulated (P < 0.001). The survival rate of patients in the miR-575 high expression group was significantly lower than that in the low expression group (P = 0.020). In addition, the overexpression of miR-575 promoted the proliferation, migration, and invasion of glioma cells. The results of this study suggested that miR-575 may be a new biomarker for the prognosis of glioma.</p>","PeriodicalId":19304,"journal":{"name":"NeuroMolecular Medicine","volume":" ","pages":"224-231"},"PeriodicalIF":3.9000,"publicationDate":"2022-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1007/s12017-021-08679-7","citationCount":"1","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"NeuroMolecular Medicine","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.1007/s12017-021-08679-7","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2021/7/16 0:00:00","PubModel":"Epub","JCR":"Q2","JCRName":"NEUROSCIENCES","Score":null,"Total":0}

引用次数: 1

Abstract

Glioma is a kind of brain tumor with low overall survival and treatment success rates in the advanced stage. Evidence has shown microRNA-575 (miR-575) plays an important role in the generation and development of various cancers. This study aimed to explore the function of miR-575 in the prognosis and cell biological behavior of glioma. qRT-PCR was used to evaluate the expression of miR-575 in glioma tissues and cells, Kaplan-Meier survival analysis and Cox regression analysis were used to evaluate the prognostic value. The proliferation ability of glioma cells was determined by MTT assay; the invasion and migration abilities were determined by transwell assays. Compared with normal brain tissues, the expression of miR-575 in glioma tissue cells was significantly up-regulated (P < 0.001). The survival rate of patients in the miR-575 high expression group was significantly lower than that in the low expression group (P = 0.020). In addition, the overexpression of miR-575 promoted the proliferation, migration, and invasion of glioma cells. The results of this study suggested that miR-575 may be a new biomarker for the prognosis of glioma.

查看原文

微信好友朋友圈 QQ好友复制链接

本刊更多论文

miR-575在胶质瘤中的表达改变与肿瘤细胞的增殖、迁移和侵袭有关。

胶质瘤是一种晚期总生存率低、治疗成功率低的脑肿瘤。有证据表明，microRNA-575 (miR-575)在各种癌症的发生和发展中起着重要作用。本研究旨在探讨miR-575在胶质瘤预后和细胞生物学行为中的作用。采用qRT-PCR检测miR-575在胶质瘤组织和细胞中的表达，采用Kaplan-Meier生存分析和Cox回归分析评估预后价值。MTT法测定胶质瘤细胞的增殖能力;采用transwell法测定其入侵和迁移能力。与正常脑组织相比，胶质瘤组织细胞中miR-575的表达明显上调(P

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文去求助

来源期刊

NeuroMolecular Medicine 医学-神经科学

CiteScore

7.10

自引率

0.00%

发文量

审稿时长

>12 weeks

期刊介绍： NeuroMolecular Medicine publishes cutting-edge original research articles and critical reviews on the molecular and biochemical basis of neurological disorders. Studies range from genetic analyses of human populations to animal and cell culture models of neurological disorders. Emerging findings concerning the identification of genetic aberrancies and their pathogenic mechanisms at the molecular and cellular levels will be included. Also covered are experimental analyses of molecular cascades involved in the development and adult plasticity of the nervous system, in neurological dysfunction, and in neuronal degeneration and repair. NeuroMolecular Medicine encompasses basic research in the fields of molecular genetics, signal transduction, plasticity, and cell death. The information published in NEMM will provide a window into the future of molecular medicine for the nervous system.