16S rDNA analysis of the intestinal microbes in osteoporotic rats.

IF 3.1 4区医学 Q2 Agricultural and Biological Sciences Bioscience of Microbiota, Food and Health Pub Date : 2021-01-01 Epub Date: 2021-02-23 DOI:10.12938/bmfh.2020-065

Yan Sun, Hui-Jie Zhang, Ran Chen, Hong-Bin Zhao, Wen-Hui Lee

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引用次数: 5

Abstract

This study aimed to reveal the differences in intestinal microbes in osteoporotic rats. The rats were divided into two groups: the control and OP (osteoporosis) groups (n=6). Days 0 and 70 were set as the time points. The rats in the OP group underwent bilateral ovariectomy (OVX). Differences between the control and OP groups were determined by 16S rDNA analysis. The relative abundances of OTUs and alpha/beta diversities were determined at days 0 days and 70. The abundances of Verrucomicrobia at the phylum level and Aerococcus, Coprobacillus, Veillonella, Anaerobiospirillum, Flavobacterium, Comamonadaceae, Ohtaekwangia, etc., at the genus level were found to be different between the control_70d and OP_70d groups. KEGG ontology analysis showed that the function of lipid metabolism could be related to OP. The 16S rDNA analysis in the OP rats revealed that intestinal microbes take part in the processes of OP and could affect lipid metabolism. Further study of the relationship between OP and intestinal microbes is necessary, and the prospect for intestinal microbes is a potential treatment for OP.

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骨质疏松大鼠肠道微生物16S rDNA分析。

本研究旨在揭示骨质疏松大鼠肠道微生物的差异。将大鼠分为对照组和骨质疏松症(OP)组(n=6)。以第0天和第70天为时间点。OP组大鼠行双侧卵巢切除术(OVX)。通过16S rDNA分析确定对照组与OP组之间的差异。分别在第0天和第70天测定OTUs和α / β多样性的相对丰度。在门水平上Verrucomicrobia的丰度，在属水平上Aerococcus、Coprobacillus、Veillonella、Anaerobiospirillum、Flavobacterium、Comamonadaceae、Ohtaekwangia等的丰度，在control_70d组和OP_70d组之间存在差异。KEGG本体论分析表明脂质代谢功能可能与OP有关。OP大鼠的16S rDNA分析显示肠道微生物参与了OP的过程，并能影响脂质代谢。进一步研究OP与肠道微生物之间的关系是必要的，肠道微生物的前景是OP的潜在治疗方法。

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来源期刊

Bioscience of Microbiota, Food and Health Immunology and Microbiology-Applied Microbiology and Biotechnology

CiteScore

5.50

自引率

3.20%

发文量

期刊介绍： Bioscience of Microbiota, Food and Health (BMFH) is a peer-reviewed scientific journal with a specific area of focus: intestinal microbiota of human and animals, lactic acid bacteria (LAB) and food immunology and food function. BMFH contains Full papers, Notes, Reviews and Letters to the editor in all areas dealing with intestinal microbiota, LAB and food immunology and food function. BMFH takes a multidisciplinary approach and focuses on a broad spectrum of issues.