Quantitative Temporal Viromics.

IF 8.1 1区 医学 Q1 VIROLOGY Annual Review of Virology Pub Date : 2021-09-29 Epub Date: 2021-06-15 DOI:10.1146/annurev-virology-091919-104458
Alice Fletcher-Etherington, Michael P Weekes
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引用次数: 6

Abstract

The abundance, localization, modifications, and protein-protein interactions of many host cell and virus proteins can change dynamically throughout the course of any viral infection. Studying these changes is critical for a comprehensive understanding of how viruses replicate and cause disease, as well as for the development of antiviral therapeutics and vaccines. Previously, we developed a mass spectrometry-based technique called quantitative temporal viromics (QTV), which employs isobaric tandem mass tags (TMTs) to allow precise comparative quantification of host and virus proteomes through a whole time course of infection. In this review, we discuss the utility and applications of QTV, exemplified by numerous studies that have since used proteomics with a variety of quantitative techniques to study virus infection through time.

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定量时间病毒组学。
在任何病毒感染过程中,许多宿主细胞和病毒蛋白的丰度、定位、修饰和蛋白-蛋白相互作用都可能发生动态变化。研究这些变化对于全面了解病毒如何复制和引起疾病以及开发抗病毒疗法和疫苗至关重要。此前,我们开发了一种基于质谱的技术,称为定量时间病毒组学(QTV),该技术采用等压串联质量标签(TMTs),可以在整个感染过程中对宿主和病毒蛋白质组进行精确的比较定量。在这篇综述中,我们讨论了QTV的效用和应用,以大量研究为例,这些研究使用蛋白质组学和各种定量技术来研究病毒感染的时间。
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来源期刊
CiteScore
19.40
自引率
0.90%
发文量
28
期刊介绍: The Annual Review of Virology serves as a conduit for disseminating thrilling advancements in our comprehension of viruses spanning animals, plants, bacteria, archaea, fungi, and protozoa. Its reviews illuminate novel concepts and trajectories in basic virology, elucidating viral disease mechanisms, exploring virus-host interactions, and scrutinizing cellular and immune responses to virus infection. These reviews underscore the exceptional capacity of viruses as potent probes for investigating cellular function.
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