Etiology of diarrheal disease among children under 5 years in Egypt: a high incidence of human bocavirus.

Neveen M Rizk, Sherif Abd-Elmaksoud, Tarek M Farid, Maha M A Abohashish, Ahmad Z Al-Herrawy, Ibrahim A Hamza
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引用次数: 4

Abstract

Background: Human bocavirus (HBoV) is globally distributed and associated with respiratory and enteric infections. Limited data are available about the incidence of HBoV in Egyptian children. We aimed to investigate the association of HBoV genotypes in children with diarrheal disease and also to determine the possibility of HBoV co-infections with other human enteric pathogens.

Methods: A total of 102 stool samples were collected from children under five years old with diarrhea. Samples were analyzed for the presence of HBoV by real-time PCR. HBoV positive samples were tested for adenovirus (AdV), rotavirus (RoV), parasitic helminths, and enteric protozoa.

Results: HBoV was detected in 58% of examined cases. HBoV-3 was the most prevalent genotype observed (44%; 45 of 102), followed by HBoV-2/4 (33%; 34 of 102) and HBoV-1 (30%; 31 of 102). Although the incidence of HBoV was higher in males (66.6%; 34 of 51) than females (49%; 25 of 51), the analysis showed no significant difference for HBoV between genders. The average HBoV concentrations were 5.3 × 104 GC/g in males and 1.03 × 105 GC/g in females. Among the HBoV-positive samples, the single infection of HBoV was 52.5% (31/59), while the co-infections with multiple viruses were found in 1.7% (1/59) for HBoV and AdV, 33.9% (20/59) for HBoV and RoV, and 11.9% (7/59) for HBoV, and RoV and AdV. No co-infection with parasitic helminths or enteric protozoa was found.

Conclusions: The single infection of HBoV in some children suffering from acute gastroenteritis indicated that HBoV could be the main etiologic agent of the disease. The study highlights the high incidence of HBoVs genotypes with remarkable multiple co-infections in the pre-school children in Egypt.

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埃及5岁以下儿童腹泻病的病因学:人类博卡病毒的高发。
背景:人类bocavavirus (HBoV)在全球分布,与呼吸道和肠道感染有关。关于埃及儿童HBoV发病率的数据有限。我们的目的是调查HBoV基因型与腹泻病儿童的关系,并确定HBoV与其他人类肠道病原体共同感染的可能性。方法:收集5岁以下腹泻患儿粪便标本102份。实时荧光定量PCR检测HBoV的存在。检测HBoV阳性标本的腺病毒(AdV)、轮状病毒(RoV)、寄生蠕虫和肠道原生动物。结果:HBoV检出率为58%。HBoV-3是最常见的基因型(44%;102例中有45例),其次是HBoV-2/4 (33%;102人中有34人)和HBoV-1 (30%;31 of 102)。尽管HBoV在男性中的发病率较高(66.6%;)比女性(49%;51例中有25例),分析显示HBoV在性别间无显著差异。男性HBoV平均浓度为5.3 × 104 GC/g,女性为1.03 × 105 GC/g。在HBoV阳性样本中,HBoV单一感染占52.5% (31/59),HBoV和AdV共感染占1.7% (1/59),HBoV和RoV共感染占33.9% (20/59),HBoV、RoV和AdV共感染占11.9%(7/59),未发现寄生虫和肠道原生动物共感染。结论:部分急性胃肠炎患儿单例HBoV感染,提示HBoV可能是本病的主要病原。该研究强调了hbov基因型在埃及学龄前儿童中具有显著的多重合并感染的高发病率。
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来源期刊
CiteScore
6.50
自引率
0.00%
发文量
25
审稿时长
10 weeks
期刊介绍: The journal accepts papers of original research which are not being considered for publication elsewhere and which contribute to the advancement of knowledge of Public Health at large
期刊最新文献
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