Prognostic Value of miR-137 in Children with Medulloblastoma and its Regulatory Effect on Tumor Progression.

IF 3.9 4区医学 Q2 NEUROSCIENCES NeuroMolecular Medicine Pub Date : 2022-06-01 Epub Date: 2021-08-18 DOI:10.1007/s12017-021-08684-w

Wenyuan Ji, Xuan Zhe, Lusheng Li, Yang Cheng, Xueling Zhao, Ping Liang, Chunxi Long, Jianjun Zhou

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引用次数: 1

Abstract

Medulloblastoma is a malignant tumor with high incidence and poor prognosis in adolescents and children. MicroRNA-137 (miR-137) has been found to be abnormally expressed in cancers such as pancreatic cancer. The purpose of this study is to explore the expression of miR-137 in MB and its role in cell physiological activities to determine the significance of miR-137 in the prognosis of MB. First, the expression of miR-137 in MB tissues and cell lines was analyzed by qRT-PCR. Then the Kaplan-Meier survival curve was used to analyze the significance of miR-137 expression in the prognosis, and the Cox regression model was used to explore the correlation between miR-137 expression and clinical characteristics. The effects of miR-137 on MB cell activities were analyzed by MTT assay, Transwell assays, and flow cytometry. It can be concluded from the results that the expression of miR-137 is down-regulated in MB tissues and cells. The down-regulation of miR-137 was significantly related to the poor prognosis of MB, and significantly related to clinical indicators. Up-regulated miR-137 inhibited cell proliferation, migration, invasion, and cell cycle progression, as well as induced cell apoptosis by targeting KDM1A. This study can conclude that miR-137 may be used as a prognostic biomarker of MB.

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miR-137在儿童髓母细胞瘤中的预后价值及其对肿瘤进展的调节作用。

髓母细胞瘤是一种发病率高、预后差的恶性肿瘤，多发于青少年和儿童。MicroRNA-137 (miR-137)已被发现在胰腺癌等癌症中异常表达。本研究旨在探讨miR-137在MB中的表达及其在细胞生理活动中的作用，以确定miR-137在MB预后中的意义。首先，通过qRT-PCR分析miR-137在MB组织和细胞系中的表达。然后采用Kaplan-Meier生存曲线分析miR-137表达对预后的意义，采用Cox回归模型探讨miR-137表达与临床特征的相关性。通过MTT法、Transwell法和流式细胞术分析miR-137对MB细胞活性的影响。结果表明，miR-137在MB组织和细胞中表达下调。miR-137的下调与MB的不良预后显著相关，且与临床指标显著相关。上调的miR-137通过靶向KDM1A抑制细胞增殖、迁移、侵袭和细胞周期进程，并诱导细胞凋亡。这项研究可以得出结论，miR-137可以作为MB的预后生物标志物。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

NeuroMolecular Medicine 医学-神经科学

CiteScore

7.10

自引率

0.00%

发文量

审稿时长

>12 weeks

期刊介绍： NeuroMolecular Medicine publishes cutting-edge original research articles and critical reviews on the molecular and biochemical basis of neurological disorders. Studies range from genetic analyses of human populations to animal and cell culture models of neurological disorders. Emerging findings concerning the identification of genetic aberrancies and their pathogenic mechanisms at the molecular and cellular levels will be included. Also covered are experimental analyses of molecular cascades involved in the development and adult plasticity of the nervous system, in neurological dysfunction, and in neuronal degeneration and repair. NeuroMolecular Medicine encompasses basic research in the fields of molecular genetics, signal transduction, plasticity, and cell death. The information published in NEMM will provide a window into the future of molecular medicine for the nervous system.