Chemerin promotes microangiopathy in diabetic retinopathy via activation of ChemR23 in rat primary microvascular endothelial cells.

IF 1.8 3区医学 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY Molecular Vision Pub Date : 2021-09-05 eCollection Date: 2021-01-01

Li Jun, Song Lin-Lin, Song Hui

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引用次数: 0

Abstract

Purpose: The correlation between chemerin and diabetic retinopathy (DR) has been demonstrated previously. We aimed to investigate the potential inflammatory and angiogenic roles of chemerin in DR using rat primary retinal microvascular endothelial cells (RRMECs).

Methods: RRMECs were incubated in low- and high-glucose media, and stable chemerin receptor (ChemR23) knockdown in RRMECs was established by lentiviral infection. Real-time quantitative PCR (RT-qPCR), enzyme-linked immunosorbent assay (ELISA), and western blotting were employed to investigate the mRNA and protein expression of intercellular adhesion molecule-1 (ICAM-1), vascular endothelial growth factor (VEGF), tumor necrosis factor-α (TNF-α), and the interleukin-6 receptor (IL-6R) to explore the inflammatory and angiogenic effects of chemerin. A scratch assay was employed to evaluate the effect of chemerin on RRMEC migration.

Results: Chemerin and TNF-α markedly increased the mRNA and protein expression of ICAM-1 in RRMECs (p<0.001). ChemR23 knockdown may have decreased the ICAM-1 expression under low- and high-glucose conditions (p<0.001). Even in the ChemR23-knockdown group, TNF-α significantly increased the mRNA and protein levels of ICAM-1 under low- and high-glucose conditions (p<0.001). Chemerin promoted VEGF expression under low- and high-glucose conditions. ChemR23 knockdown markedly decreased VEGF levels under low- and high-glucose conditions (p<0.05) and significantly decreased RRMEC migration (p<0.001).

Conclusions: Chemerin promotes the expression of ICAM-1, the secretion of VEGF, and the migration of RRMECs via the activation of ChemR23.

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Chemerin通过激活大鼠原代微血管内皮细胞中的ChemR23促进糖尿病视网膜病变的微血管病变。

目的:趋化素与糖尿病视网膜病变(DR)的相关性已被证实。我们的目的是利用大鼠原代视网膜微血管内皮细胞(RRMECs)研究趋化素在DR中的潜在炎症和血管生成作用。方法:在低糖和高糖培养基中培养RRMECs，通过慢病毒感染建立RRMECs稳定的趋化素受体(ChemR23)敲低。采用实时荧光定量PCR (RT-qPCR)、酶联免疫吸附法(ELISA)和western blotting检测细胞间粘附分子-1 (ICAM-1)、血管内皮生长因子(VEGF)、肿瘤坏死因子-α (TNF-α)和白细胞介素-6受体(IL-6R) mRNA和蛋白的表达，探讨趋化素的炎症和血管生成作用。采用划痕法评价趋化素对RRMEC迁移的影响。结果:Chemerin和TNF-α显著提高了RRMECs中ICAM-1 mRNA和蛋白的表达(p结论:Chemerin通过激活ChemR23促进ICAM-1的表达、VEGF的分泌和RRMECs的迁移。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Molecular Vision 生物-生化与分子生物学

CiteScore

4.40

自引率

0.00%

发文量

审稿时长

1 months

期刊介绍： Molecular Vision is a peer-reviewed journal dedicated to the dissemination of research results in molecular biology, cell biology, and the genetics of the visual system (ocular and cortical). Molecular Vision publishes articles presenting original research that has not previously been published and comprehensive articles reviewing the current status of a particular field or topic. Submissions to Molecular Vision are subjected to rigorous peer review. Molecular Vision does NOT publish preprints. For authors, Molecular Vision provides a rapid means of communicating important results. Access to Molecular Vision is free and unrestricted, allowing the widest possible audience for your article. Digital publishing allows you to use color images freely (and without fees). Additionally, you may publish animations, sounds, or other supplementary information that clarifies or supports your article. Each of the authors of an article may also list an electronic mail address (which will be updated upon request) to give interested readers easy access to authors.