Refraining from use diminishes cannabis-associated epigenetic changes in human sperm.

IF 5.5 3区材料科学 Q2 CHEMISTRY, PHYSICAL ACS Applied Energy Materials Pub Date : 2021-09-21 eCollection Date: 2021-01-01 DOI:10.1093/eep/dvab009

Rose Schrott, Susan K Murphy, Jennifer L Modliszewski, Dillon E King, Bendu Hill, Nilda Itchon-Ramos, Douglas Raburn, Thomas Price, Edward D Levin, Ryan Vandrey, David L Corcoran, Scott H Kollins, John T Mitchell

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Abstract

Cannabis use alters sperm DNA methylation, but the potential reversibility of these changes is unknown. Semen samples from cannabis users and non-user controls were collected at baseline and again following a 77-day period of cannabis abstinence (one spermatogenic cycle). Users and controls did not significantly differ by demographics or semen analyses. Whole-genome bisulfite sequencing identified 163 CpG sites with significantly different DNA methylation in sperm between groups (P < 2.94 × 10^-9). Genes associated with altered CpG sites were enriched with those involved in development, including cardiogenesis and neurodevelopment. Many of the differences in sperm DNA methylation between groups were diminished after cannabis abstinence. These results indicate that sustained cannabis abstinence significantly reduces the number of sperm showing cannabis-associated alterations at genes important for early development.

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不使用大麻会减少人类精子中与大麻相关的表观遗传变化。

使用大麻会改变精子DNA甲基化，但这些变化的潜在可逆性尚不清楚。在基线时收集大麻使用者和非使用者对照的精液样本，并在77天的大麻戒断期（一个生精周期）后再次收集。使用者和对照组在人口统计学或精液分析方面没有显著差异。亚硫酸氢盐全基因组测序鉴定出163个CpG位点在两组精子中DNA甲基化有显著差异（P -9）。与CpG位点改变相关的基因与发育相关的基因丰富，包括心脏发生和神经发育。在戒掉大麻后，两组之间精子DNA甲基化的许多差异都消失了。这些结果表明，持续的大麻戒断显着减少精子的数量，显示大麻相关的基因改变对早期发育很重要。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

ACS Applied Energy Materials Materials Science-Materials Chemistry

CiteScore

10.30

自引率

6.20%

发文量

1368

期刊介绍： ACS Applied Energy Materials is an interdisciplinary journal publishing original research covering all aspects of materials, engineering, chemistry, physics and biology relevant to energy conversion and storage. The journal is devoted to reports of new and original experimental and theoretical research of an applied nature that integrate knowledge in the areas of materials, engineering, physics, bioscience, and chemistry into important energy applications.