Effect of peroxisome proliferator-activated receptor-γ coactivator-1α on liver injury induced by periodontitis in rats.

Q3 Medicine 华西口腔医学杂志 Pub Date : 2021-10-01 DOI:10.7518/hxkq.2021.05.004

Bo-Yuan Xia, Yan Li, Xu Ding, Xin Li, Xin-Chan Liu, Wei-Xian Yu

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Abstract

Objectives: To investigate the effect of peroxisome proliferator-activated receptor-γ coactivator-1α (PGC-1α) on liver injury induced by periodontitis in rats.

Methods: Twenty-four male Wistar rats were randomly divided into two groups: control group and periodontitis group, twelve per group. In periodontitis group, the periodontitis models were established for the maxillary first molars in rats by means of "wire ligation+vaccinationwith Porphyromonas gingivalis", the control group was inoculated with the equal volume of 2% sodium carboxymethyl cellulose in the same position, for 6 weeks. The probing depth, tooth mobility and sulcus bleeding index were detected. Hematoxylin-eosin (HE) staining was used to observe the pathological changes of liver tissues in rats. The quantitative real-time polymerase chain reaction (qRT-PCR) and immunohistochemistry (IHC) were used to detect the gene and protein expression levels of PGC-1α, nuclear factor erythroid 2-related factor 2 (Nrf2) and mitochondrial transcription factor A (TFAM) in liver tissues of rats.

Results: The probing depth, tooth mobility and sulcus bleeding index in periodontitis group were significantly higher than that in control group. HE staining showed in periodontitis group, hepatic cords ranged disorderly and there were vacuoles in cells and inflammatory cells infiltrated in liver tissues of rats, and there was no obvious abnormality in control group. The qRT-PCR results showed that the mRNA expression levels of Pgc-1α, Nrf2 and Tfam in liver tissues of rats in periodontitis group were lower obviously than that in control group. IHC results showed that the protein expression level of PGC-1α in liver tissues of rats in periodontitis group was decreased significantly than that in control group.

Conclusions: PGC-1α may be involved in the process of periodontitis-induced liver injury in rats.

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过氧化物酶体增殖物激活受体-γ共激活因子-1α对大鼠牙周炎肝损伤的影响。

目的:探讨过氧化物酶体增殖物激活受体-γ共激活因子-1α (PGC-1α)对大鼠牙周炎肝损伤的影响。方法:24只雄性Wistar大鼠随机分为对照组和牙周炎组，每组12只。牙周炎组采用“金属丝结扎+接种牙龈卟啉单胞菌”的方法建立大鼠上颌第一磨牙牙周炎模型，对照组在相同位置接种等体积的2%羧甲基纤维素钠，接种6周。检测探牙深度、牙活动性及牙沟出血指数。采用苏木精-伊红(HE)染色法观察大鼠肝组织的病理变化。采用实时荧光定量聚合酶链式反应(qRT-PCR)和免疫组化(IHC)技术检测大鼠肝组织中PGC-1α、核因子-红细胞2相关因子2 (Nrf2)和线粒体转录因子A (TFAM)的基因和蛋白表达水平。结果:牙周炎组探诊深度、牙活动性、牙沟出血指数均显著高于对照组。HE染色显示，牙周炎组大鼠肝索排列紊乱，细胞内可见空泡，肝组织内可见炎性细胞浸润，对照组无明显异常。qRT-PCR结果显示，牙周炎组大鼠肝组织中Pgc-1α、Nrf2、Tfam mRNA表达水平明显低于对照组。免疫组化结果显示，牙周炎组大鼠肝组织中PGC-1α蛋白表达水平明显低于对照组。结论:PGC-1α可能参与了大鼠牙周炎所致肝损伤的过程。

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来源期刊

华西口腔医学杂志 Medicine-Medicine (all)

CiteScore

0.80

自引率

0.00%

发文量

6397

期刊介绍： West China Journal of Stomatology (WCJS, pISSN 1000-1182, eISSN 2618-0456, CN 51-1169/R), published bimonthly, is a peer-reviewed Open Access journal, hosted by Sichuan university and Ministry of Education of the People's Republic of China. WCJS was established in 1983 and indexed in Medline/Pubmed, SCOPUS, EBSCO, Chemical Abstract(CA), CNKI, WANFANG Data, etc.