Effects of different irradiators on the establishment of osteoradionecrosis model of rat mandible.

Q3 Medicine 华西口腔医学杂志 Pub Date : 2021-10-01 DOI:10.7518/hxkq.2021.05.005

Hua-Wei Chen, Sheng-Fu Zhang, Hai-Tao He

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Abstract

Objectives: To compare the effects of different irradiators on the establishment of osteoradionecrosis of jaw model (ORNJ) to explore an ideal modeling method.

Methods: A total of 33 adult SD rats were included and randomly divided into three groups according to the radiation equipment, namely, the blank control (CN, 3 rats), group A (linear accelerator irradiation, 15 rats), and group B (small-animal irradiator irradiation, 15 rats). Groups A and B were irradiated with daily fractions of 7, 8, and 9 Gy for 5 days and further divided into three subgroups as follows: group A₃₅/B₃₅, 35 Gy; group A₄₀/B₄₀, 40 Gy; and group A₄₅/B₄₅, 45 Gy. The left mandibular molars of the rats were extracted 1 week after irradiation. The rats were sacrificed 3 weeks after tooth extraction, and the mandible specimens were obtained for gross observation, micro-CT scanning, and histological detection to evaluate the success rate of modeling.

Results: At 3 weeks after dental extractions, complete gingival healing was found in the regions of dental extractions in groups A₃₅ and A₄₀. However, failed gingival healing and bone exposure were found in groups A₄₅ and B. Hematoxylin and eosin staining showed necrotic bone of the irradiated mandible in groups A₄₀, A₄₅,and B, with success modeling rates of 40% in group A and 93.3% in group B.

Conclusions: Small-animal irradiator irradiation is an ideal device for establishing ORNJ model.

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不同辐照剂对大鼠下颌骨放射性坏死模型建立的影响。

目的:比较不同照射方式对建立颌骨放射性坏死模型(ORNJ)的影响，探索理想的造模方法。方法:选取成年SD大鼠33只，根据照射设备随机分为空白对照(CN, 3只大鼠)、A组(直线加速器照射，15只大鼠)、B组(小动物照射器照射，15只大鼠)。A、B组分别按每日7、8、9 Gy剂量照射5 d，再分为3个亚组:A35/B35组，35 Gy;A40/B40组，40 Gy;A45/B45组，45 Gy。照射后1周取出大鼠左下颌磨牙。拔牙后3周处死大鼠，取下颌骨标本进行大体观察、显微ct扫描和组织学检测，评估造模成功率。结果:拔牙后3周，A35组和A40组拔牙部位牙龈完全愈合。然而，A45组和B组牙龈愈合和骨暴露均失败。苏木精和伊红染色显示，A40组、A45组和B组辐照下颌骨骨坏死，A组和B组造模成功率分别为40%和93.3%。结论:小动物辐照是建立ORNJ模型的理想装置。

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来源期刊

华西口腔医学杂志 Medicine-Medicine (all)

CiteScore

0.80

自引率

0.00%

发文量

6397

期刊介绍： West China Journal of Stomatology (WCJS, pISSN 1000-1182, eISSN 2618-0456, CN 51-1169/R), published bimonthly, is a peer-reviewed Open Access journal, hosted by Sichuan university and Ministry of Education of the People's Republic of China. WCJS was established in 1983 and indexed in Medline/Pubmed, SCOPUS, EBSCO, Chemical Abstract(CA), CNKI, WANFANG Data, etc.