The complete mitochondrial genome of Ophiocordyceps gracilis and its comparison with related species.

IF 5.2 1区生物学 Q1 MYCOLOGY Ima Fungus Pub Date : 2021-10-20 DOI:10.1186/s43008-021-00081-z

Aifeire Abuduaini, Yuan-Bing Wang, Hui-Ying Zhou, Rui-Ping Kang, Ming-Liang Ding, Yu Jiang, Fei-Ya Suo, Luo-Dong Huang

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引用次数: 5

Abstract

In this study, the complete mitochondrial genome of O. gracilis was sequenced and assembled before being compared with related species. As the second largest mitogenome reported in the family Ophiocordycipitaceae, the mitogenome of O. gracilis (voucher OG201301) is a circular DNA molecule of 134,288 bp that contains numerous introns and longer intergenomic regions. UCA was detected as anticodon in tRNA-Sec of O. gracilis, while comparative mitogenome analysis of nine Ophiocordycipitaceae fungi indicated that the order and contents of PCGs and rRNA genes were considerably conserved and could descend from a common ancestor in Ophiocordycipitaceae. In addition, the expansion of mitochondrial organization, introns, gene length, and order of O. gracilis were determined to be similar to those of O. sinensis, which indicated common mechanisms underlying adaptive evolution in O. gracilis and O. sinensis. Based on the mitochondrial gene dataset (15 PCGs and 2 RNA genes), a close genetic relationship between O. gracilis and O. sinensis was revealed through phylogenetic analysis. This study is the first to investigate the molecular evolution, phylogenetic pattern, and genetic structure characteristics of mitogenome in O. gracilis. Based on the obtained results, the mitogenome of O. gracilis can increase understanding of the genetic diversity and evolution of cordycipitoid fungi.

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麻虫草的线粒体全基因组及其与近缘种的比较。

在本研究中，在与近缘种进行比较之前，对薄叶菊的线粒体全基因组进行了测序和组装。作为Ophiocordycipitaceae家族中报道的第二大有丝分裂基因组，O. gracilis(编号OG201301)的有丝分裂基因组是一个134,288 bp的环状DNA分子，包含大量内含子和更长的基因组间区域。UCA作为抗密码子存在于O. gracilis的tRNA-Sec中，而9种蛇虫草科真菌的有丝分裂基因组比较分析表明，PCGs和rRNA基因的序列和含量相当保守，可能来自蛇虫草科的一个共同祖先。此外,组织线粒体的扩张,内含子,基因长度,和秩序的欧股薄肌测定o .的类似,这表明共同进化机制适应欧股薄肌和o .制成。基于线粒体基因数据集(15个PCGs基因和2个RNA基因)，通过系统发育分析，揭示了薄叶金龟与中华金龟的亲缘关系。本研究首次探讨了薄叶菊有丝分裂基因组的分子进化、系统发育模式和遗传结构特征。研究结果表明，该真菌有丝分裂基因组的研究有助于进一步了解虫草类真菌的遗传多样性和进化过程。

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来源期刊

Ima Fungus Agricultural and Biological Sciences-Agricultural and Biological Sciences (miscellaneous)

CiteScore

11.00

自引率

3.70%

发文量

审稿时长

20 weeks

期刊介绍： The flagship journal of the International Mycological Association. IMA Fungus is an international, peer-reviewed, open-access, full colour, fast-track journal. Papers on any aspect of mycology are considered, and published on-line with final pagination after proofs have been corrected; they are then effectively published under the International Code of Nomenclature for algae, fungi, and plants. The journal strongly supports good practice policies, and requires voucher specimens or cultures to be deposited in a public collection with an online database, DNA sequences in GenBank, alignments in TreeBASE, and validating information on new scientific names, including typifications, to be lodged in MycoBank. News, meeting reports, personalia, research news, correspondence, book news, and information on forthcoming international meetings are included in each issue