Natural killer cell deficiency experiences higher risk of sepsis after critical intracerebral hemorrhage.

Abstract

Background: Lymphopenia is common in intracerebral hemorrhage (ICH) and may predispose to severe infections such as sepsis. However, what specific kind of lymphocytes subsets decreases is still unclear. We investigated the impact of lymphocytes subsets on post-critical ICH infections and mortality.

Methods: Consecutive ICH patients (admitted to a single center between January 2017 and January 2018) were prospectively assessed to evaluate the following main parameters: peripheral blood lymphocytes, infections, and clinical scores. Predicting factors of sepsis were measured using multivariate Logistic regressions analysis. A Kaplan-Meier survival curve was performed to compare the mortality between septic and nonseptic patients. Survival status was evaluated by multivariate Cox regression analysis.

Results: In total, 112 critical ICH cases were enrolled including 29 septic patients. Total counts of lymphocytes decreased accordingly with reduced lymphocyte subsets, especially natural killer (NK) cells and CD8⁺T lymphocytes after ICH. Septic patients had a higher incidence of pneumonia, a longer length of stay, higher 90-day mortality, and worse long-term outcomes. Multivariate Logistic regression analysis showed venous catheterization, high APACHE-II score (>15), low GCS score (3-5), and NK cells percentages on admission were independently associated with ensuing sepsis. After sepsis, the percentages of CD4+T and NK cells percentages decreased, CD8+T cells increased followed by a significantly decreased CD4/CD8 ratio. Bloodstream infection alone directly affected the survival status of patients with sepsis.

Conclusions: Critical ICH patients underwent immune dysfunction and NK cells deficiency could favor nosocomial threatening sepsis after ICH.