In vitro effects of pentoxifylline and coenzyme Q10 on the sperm of oligoasthenoteratozoospermia patients.

IF 2.1 4区医学 Q2 OBSTETRICS & GYNECOLOGY Human Fertility Pub Date : 2023-12-01 Epub Date: 2021-12-21 DOI:10.1080/14647273.2021.2017024

Mohammad Jafar Rezaie, Azra Allahveisi, Amir Raoofi, Masomeh Rezaei, Bahram Nikkhoo, Amin Mousavi Khaneghah

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引用次数: 2

Abstract

The effect of in-vitro sperm incubation with Pentoxifylline (PTX) and Coenzyme Q10 (CoQ10) in Oligoasthenoteratozoospermia (OAT) patients was evaluated. Semen samples were obtained from men with Normozoospermia and men with OAT. Motile sperm from the two groups were subdivided into four subgroups: (i) without incubation with PTX + CoQ10; (ii) incubation with PTX; (iii) Incubation with CoQ10; and (iv) incubation with a combination of PTX + CoQ10. Then, sperm parameters, chromatin, DNA and membrane integrity, protamine deficiency, apoptosis, mitochondrial activity, sperm chromatin dispersion test (SCD), hypo-osmotic swelling test (HOS), chromomycin A3 (CMA3), Terminal deoxynucleotidyl transferase dUTP nick end labelling (TUNEL), and diaminobenzidine (DAB) assays were evaluated, respectively. Sperm incubated with CoQ10 and a combination of CoQ10 and PTX resulted in a significant increase in the sperm parameters. Also, a significant decrease was noted with a combination of PTX and CoQ10 in normal men. There was a significant difference between CoQ10 treated and CoQ10 + PTX treated groups in comparison with the OAT group in the percentage of the DNA fragmentation, sperm apoptosis, AB+, HOS test + and sperm mitochondrial activity. Incubated sperm with CoQ10, PTX, and in combination with each other can improve sperm parameters in OAT patients.

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喷托维林和辅酶Q10对少精症患者精子的体外影响

本研究评估了用五氧化锡（Pentoxifylline，PTX）和辅酶Q10（CoQ10）对少精子症（Oligoasthenoteratozoospermia，OAT）患者的精子进行体外培养的效果。研究人员从患有正常无精子症的男性和患有 OAT 的男性中获取精液样本。两组患者的活动精子被细分为四个亚组：(i) PTX + CoQ10 未孵育组；(ii) PTX 孵化组；(iii) CoQ10 孵化组；(iv) PTX + CoQ10 组合孵育组。然后，分别对精子参数、染色质、DNA和膜完整性、原胺缺乏、细胞凋亡、线粒体活性、精子染色质分散试验（SCD）、低渗肿胀试验（HOS）、色霉素 A3（CMA3）、末端脱氧核苷酸转移酶 dUTP 缺口标记（TUNEL）和二氨基联苯胺（DAB）检测进行评估。用 CoQ10 以及 CoQ10 和 PTX 的组合培养精子可显著提高精子参数。此外，正常男性的精子在 PTX 和 CoQ10 的联合作用下会明显减少。与 OAT 组相比，CoQ10 处理组和 CoQ10 + PTX 处理组在 DNA 断裂百分比、精子凋亡、AB+、HOS 测试 + 和精子线粒体活性方面存在明显差异。用 CoQ10、PTX 以及它们之间的组合培养精子可以改善 OAT 患者的精子参数。

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来源期刊

Human Fertility OBSTETRICS & GYNECOLOGY-REPRODUCTIVE BIOLOGY

CiteScore

3.30

自引率

5.30%

发文量

期刊介绍： Human Fertility is a leading international, multidisciplinary journal dedicated to furthering research and promoting good practice in the areas of human fertility and infertility. Topics included span the range from molecular medicine to healthcare delivery, and contributions are welcomed from professionals and academics from the spectrum of disciplines concerned with human fertility. It is published on behalf of the British Fertility Society. The journal also provides a forum for the publication of peer-reviewed articles arising out of the activities of the Association of Biomedical Andrologists, the Association of Clinical Embryologists, the Association of Irish Clinical Embryologists, the British Andrology Society, the British Infertility Counselling Association, the Irish Fertility Society and the Royal College of Nursing Fertility Nurses Group. All submissions are welcome. Articles considered include original papers, reviews, policy statements, commentaries, debates, correspondence, and reports of sessions at meetings. The journal also publishes refereed abstracts from the meetings of the constituent organizations.