Identification and Validation of Hub Genes Predicting Prognosis of Hepatocellular Carcinoma.

IF 1.8 3区医学 Q3 GASTROENTEROLOGY & HEPATOLOGY Digestive Surgery Pub Date : 2022-01-01 Epub Date: 2021-11-17 DOI:10.1159/000520893

Ziyan Chen, Haitao Yu, Lijun Wu, Sina Zhang, Zhihui Lin, Tuo Deng, Bangjie He, Zhengping Yu, Gang Chen, Fang Wu

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引用次数: 2

Abstract

Introduction: The aim of this study is selecting the hub genes associated with hepatocellular carcinoma (HCC) to construct a Cox regression model for predicting prognosis in HCC patients.

Methods: Using HCC patient data from the ICGC and TCGA databases, screened for 40 core genes highly correlated with histological grade of HCC. Univariate and multivariate Cox regression analyses were performed on the genes highly associated with HCC prognosis, and the model was established. The expression of those genes was measured by immunohistochemistry in 110 HCC patients who underwent the surgery in the First Affiliated Hospital of Wenzhou Medical University. The survival of HCC patients was analyzed by the Kaplan-Meier method.

Results: Eight genes (CDC45, CENPA, MCM10, MELK, CDC20, ASF1B, FANCD2, and NCAPH) were correlated with prognosis, and the same result was observed in 110 HCC patients. Using the regression model, the HCC patients in the training set were classified as high- and low-risk groups. The overall survival of patients in the high-risk group was shorter than that in the low-risk group, and the same results were obtained in the verification set.

Conclusion: This study found that the risk model according to these 8 genes can be used as a predictor of prognosis in HCC. These genes may become alternative biomarkers and therapeutic targets and provide new therapeutic strategies for HCC.

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预测肝细胞癌预后的枢纽基因的鉴定和验证。

本研究的目的是选择与肝细胞癌(HCC)相关的枢纽基因，构建预测HCC患者预后的Cox回归模型。方法:利用ICGC和TCGA数据库中的HCC患者数据，筛选与HCC组织学分级高度相关的40个核心基因。对HCC预后高度相关基因进行单因素和多因素Cox回归分析，建立模型。在温州医科大学第一附属医院行肝细胞癌手术的110例HCC患者中，用免疫组织化学方法检测了这些基因的表达。采用Kaplan-Meier法分析HCC患者的生存期。结果:8个基因(CDC45、CENPA、MCM10、MELK、CDC20、ASF1B、FANCD2、NCAPH)与预后相关，在110例HCC患者中观察到相同的结果。利用回归模型将训练集中的HCC患者分为高危组和低危组。高危组患者总生存期短于低危组，验证集中结果相同。结论:本研究发现基于这8个基因的风险模型可作为HCC预后的预测因子。这些基因可能成为替代的生物标志物和治疗靶点，并为HCC提供新的治疗策略。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Digestive Surgery 医学-外科

CiteScore

4.90

自引率

3.70%

发文量

审稿时长

3 months

期刊介绍： ''Digestive Surgery'' presents a comprehensive overview in the field of gastrointestinal surgery. Interdisciplinary in scope, the journal keeps the specialist aware of advances in all fields that contribute to improvements in the diagnosis and treatment of gastrointestinal disease. Particular emphasis is given to articles that evaluate not only recent clinical developments, especially clinical trials and technical innovations such as new endoscopic and laparoscopic procedures, but also relevant translational research. Each contribution is carefully aligned with the need of the digestive surgeon. Thus, the journal is an important component of the continuing medical education of surgeons who want their practice to benefit from a familiarity with new knowledge in all its dimensions.