Ning Wei , Ziqiang Wu , Dong Lu , Jingkun Xiao , Chunze Zhou , Senlin Chu , Weifu Lv
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引用次数: 0
Abstract
Objective
This study aimed to analyze the effects of transcatheter arterial embolization (TAE) combined with portal venous embolization (PVE) on the expression of MMP-2 in residual VX2 liver tumor tissues, liver function and non-embolic lobe regeneration.
Methods
A total of 72 rabbits were randomly divided into Sham, TAE, PVE and TAE + PVE groups (n = 18/group). The tissue samples from each group were taken at 6 h, 3 days and 7 days after interventional operation, respectively. MMP-2 expression was detected by immunohistochemistry, Real-time PCR, and Western-blotting. The main indicators (such as AST, ATL, and TBIL) of liver function and the volume of non-embolized hepatic lobes were measured in each group after operation. One-way ANOVA and Kruskal-wallis method were used for statistical analysis.
Results
The expression of MMP-2 mRNA and protein remained the highest in the Sham group, and the expression of MMP-2 mRNA and protein in TAE, PVE and TAE + PVE groups were successively increased, and the expression of MMP-2 in TAE + PVE group was always significantly higher than TAE group. The AST and ALT levels in each group on day 7 after operation showed a significant declination, and all groups have recovered to the preoperative baseline level and TBIL has a slight fluctuation in each group after operation with no statistical difference. On day 7 after operation, the increasing volume of non-embolized liver lobes in TAE + PVE group showed a more significant effect than those in PVE group, but there was no statistical significance (37.62 ± 1.54 ml VS 36.18 ± 1.15 ml, P = 0.881), and its volume was significantly higher than those in the sham group (27.03 ± 1.11 ml).
Conclusion
TAE + PVE is considered to be an efficient and safe approach for treating rabbit VX2 liver transplantation tumor, but the expression of MMP-2 increased fastest after TAE + PVE, which might promote tumor cell invasion and metastasis.