The effect of valproic acid on SLC5A8 expression in gonad-intact and gonadectomized rat thymocytes.

IF 3 3区医学 Q3 IMMUNOLOGY International Journal of Immunopathology and Pharmacology Pub Date : 2022-01-01 DOI:10.1177/20587384211051954

Milda Juknevičienė, Ingrida Balnytė, Angelija Valančiūtė, Jūratė Stanevičiūtė, Kęstutis Sužiedėlis, Donatas Stakišaitis

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Abstract

Background: Valproic acid (VPA) pharmacological mechanisms are related to the anti-inflammatory and anti-viral effects. VPA is a histone deacetylases inhibitor and serves a role in its immunomodulatory impacts. VPA has complex effects on immune cell's mitochondrial metabolism. The SLC5A8 transporter of short fatty acids has an active role in regulating mitochondrial metabolism. The study aimed to investigate whether SLC5A8 expresses the sex-related difference and how SLC5A8 expression depends on gonadal hormones, VPA treatment, and NKCC1 expression in rat thymocytes.

Methods: Control groups and VPA-treated gonad-intact and gonadectomized Wistar male and female rats were investigated (n = 6 in a group). The VPA 300 mg/kg/day in drinking water was given for 4 weeks. The SLC5A8 (Slc5a8 gene) and NKCC1 (Slc12a2 gene) RNA expressions were determined by the RT-PCR method.

Results: The higher Slc5a8 expression was found in the gonad-intact males than respective females (p = 0.004). VPA treatment decreased the Slc5a8 expression in gonad-intact and castrated males (p = 0.02 and p = 0.03, respectively), and increased in gonad-intact female rats compared to their control (p = 0.03). No significant difference in the Slc5a8 expression between the ovariectomized female control and VPA-treated females was determined (p > 0.05). VPA treatment alters the correlation between Slc5a8 and Slc12a2 gene expression in thymocytes of gonad-intact rats.

Conclusion: VPA effect on the Slc5a8 expression in rat thymocytes is gender- and gonadal hormone-dependent.

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丙戊酸对无性腺和性腺切除大鼠胸腺细胞中 SLC5A8 表达的影响

背景：丙戊酸（VPA）的药理机制与抗炎和抗病毒作用有关。VPA 是一种组蛋白去乙酰化酶抑制剂，具有免疫调节作用。VPA 对免疫细胞的线粒体代谢有复杂的影响。短脂肪酸的 SLC5A8 转运体在调节线粒体代谢方面发挥着积极作用。本研究旨在探讨大鼠胸腺细胞中 SLC5A8 的表达是否存在性别差异，以及 SLC5A8 的表达如何依赖于性腺激素、VPA 处理和 NKCC1 的表达：方法：研究对象为对照组和经 VPA 处理的性腺未受损和性腺切除的 Wistar 雄性和雌性大鼠（每组 6 只）。VPA 300 毫克/千克/天，连续 4 周。采用 RT-PCR 方法检测 SLC5A8（Slc5a8 基因）和 NKCC1（Slc12a2 基因）的 RNA 表达：结果：未触及性腺的雄性动物的 Slc5a8 表达量高于雌性动物（p = 0.004）。与对照组相比，VPA 处理降低了性腺未受影响的雄性大鼠和阉割雄性大鼠的 Slc5a8 表达（p = 0.02 和 p = 0.03），增加了性腺未受影响的雌性大鼠的 Slc5a8 表达（p = 0.03）。卵巢切除的雌性对照组与经 VPA 处理的雌性对照组之间的 Slc5a8 表达无明显差异（p > 0.05）。VPA改变了性腺未受损大鼠胸腺细胞中Slc5a8和Slc12a2基因表达的相关性：结论：VPA 对大鼠胸腺细胞中 Slc5a8 表达的影响与性别和性腺激素有关。

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来源期刊

International Journal of Immunopathology and Pharmacology 医学-病理学

CiteScore

4.00

自引率

0.00%

发文量

审稿时长

15 weeks

期刊介绍： International Journal of Immunopathology and Pharmacology is an Open Access peer-reviewed journal publishing original papers describing research in the fields of immunology, pathology and pharmacology. The intention is that the journal should reflect both the experimental and clinical aspects of immunology as well as advances in the understanding of the pathology and pharmacology of the immune system.