Efficacy of dispersed fibrous carbon sorbent in treatment of malignant fungating wounds.

Q3 Medicine Experimental oncology Pub Date : 2021-12-01 DOI:10.32471/exp-oncology.2312-8852.vol-43-no-4.16757

L A Sakhno, L P Babenko, L M Lazarenko, V G Korotych, V V Sarnatskaya, E A Snezhkova, M Ya Spivak, V G Nikolaev

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Abstract

Aim: To evaluate the influence of dispersed fibrous carbon sorbent (DFCS) on malignant fungating wounds (MFWs) in Guerin's carcinoma-bearing rats with an assessment of wound bacterial microflora.

Materials and methods: The study was performed on female Wistar rats inoculated subcutaneously with Guerin T8 carcinoma into the upper interscapular region. On day 20, the tumors were infected by injecting the suspension of S. aureus 8325-4. After 24 h, gauze dressings were applied daily to the wounds in the control group and DFCS in the experimental one. The state of the wounds was assessed by visual control with photo monitoring, odor control on a verbal rating scale and microbiological analysis of qualitative and quantitative characteristics of wound microbiota.

Results: On the 3rd day after the start of dressings, the number of S. aureus of 8325-4 strain was significantly higher in the wounds of rats in control group than experimental one (5.14 ± 0.27 vs 3.43 ± 0.33 lg CFU/ml). The total number of Staphylococci in the DFCS-treated wounds did not differ statistically from the number of S. aureus 8325-4 strain whereas in control ones were higher by an order of magnitude. On the 10th day the total number of Staphylococci and S. aureus 8325-4 in particular, remained consistently high in the wounds of the control rats, while was almost five orders of magnitude lower and represented mainly by S. aureus 8325-4 in the experimental ones. The total number of aerobic and optionally anaerobic microorganisms was significantly lower as well. A greater variety of microorganisms in the gauze-covered wounds, as well as an their increased number were accompanied by enhancement of the wound odor from "noticeable" to "strong". There was an appearance of a "barely noticeable" odor in only one animal from the experimental group.

Conclusion: The study has demonstrated the ability of DFCS to control substantially the bacterial microflora as well as malodor of MWFs in vivo. The results obtained can contribute to solving the problem of improving the quality of palliative care for patients with malignant and other chronic wounds.

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分散纤维炭吸附剂治疗恶性真菌性创面的疗效。

目的:评价分散纤维炭吸附剂(DFCS)对格林癌大鼠恶性真菌性创面(MFWs)的影响，并对创面菌群进行评价。材料与方法:采用雌性Wistar大鼠上肩胛间区皮下接种Guerin T8癌。第20天，注射金黄色葡萄球菌8325-4悬液感染肿瘤。24h后，对照组创面每日涂抹纱布敷料，实验组创面每日涂抹DFCS敷料。通过视觉控制与光监测、气味控制(口头评定量表)和微生物学分析伤口微生物群的定性和定量特征来评估伤口的状态。结果:在敷料开始后第3天，对照组大鼠创面中83225 -4菌株金黄色葡萄球菌数量明显高于实验组(5.14±0.27 vs 3.43±0.33 lg CFU/ml)。dfcs处理的伤口中葡萄球菌总数与金黄色葡萄球菌8325-4菌株的数量无统计学差异，而对照组的葡萄球菌总数则高出一个数量级。在第10天，对照组大鼠伤口中葡萄球菌和金黄色葡萄球菌8325-4的总数一直保持在较高水平，而实验大鼠伤口中葡萄球菌和金黄色葡萄球菌8325-4的数量则下降了近5个数量级，主要以金黄色葡萄球菌8325-4为主。好氧微生物和可选厌氧微生物的总数也显著降低。在纱布覆盖的伤口中，微生物种类越来越多，数量也越来越多，伤口气味也从“明显”增强到“强烈”。实验组中只有一只动物出现了“几乎不明显”的气味。结论:本研究证明了DFCS在体内对MWFs的细菌菌群和恶臭有明显的控制作用。所得结果有助于解决提高恶性及其他慢性创伤患者姑息治疗质量的问题。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Experimental oncology Medicine-Oncology

CiteScore

1.40

自引率

0.00%

发文量

期刊介绍： The Experimental Oncology is an English-language journal that publishes review articles, original contributions, short communications, case reports and technical advances presenting new data in the field of experimental and fundamental oncology. Manuscripts should be written in English, contain original work, which has not been published or submitted for publication elsewhere. It also implies the transfer of the Copyright from the author to “Experimental Oncology”. No part of journal publications may be reproduced, stored in a retrieval system or transmitted in any form or by any means without the prior permission of the publisher.