Gravity induces asymmetric Ca2+ spikes in the root cap in the early stage of gravitropism.

Abstract

Gravitropism is an important strategy for the adaptation of plants to the changing environment. Previous reports indicated that Ca²⁺ participated in plant gravity response. However, present information on the functions of Ca²⁺ in plant gravitropism was obtained mainly on coleoptiles, hypocotyls, and petioles, little is known about the dynamic changes of Ca²⁺ during root gravitropism. In the present study, the transgenic Arabidopsis thaliana R-GECO1 was placed horizontally and subsequently vertically on a refitted Leica SP8 laser scanning confocal microscopy with a vertical stage. Real-time observations indicated that gravistimulation induced not only an increase in the Ca²⁺ concentration, but also an accelerated occurrence of Ca²⁺ sparks in the root cap, especially in the lower side of the lateral root cap, indicating a strong tie between Ca²⁺ dynamics and gravistimulation during the early stage of root gravity response.