Serum Neurofilament Light as a Biomarker of Traumatic Brain Injury in the Presence of Concomitant Peripheral Injury.

IF 3.4 Q2 MEDICINE, RESEARCH & EXPERIMENTAL Biomarker Insights Pub Date : 2021-10-26 eCollection Date: 2021-01-01 DOI:10.1177/11772719211053449
Ker Rui Wong, William T O'Brien, Mujun Sun, Glenn Yamakawa, Terence J O'Brien, Richelle Mychasiuk, Sandy R Shultz, Stuart J McDonald, Rhys D Brady
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引用次数: 8

Abstract

Introduction: Serum neurofilament light (NfL) is an emerging biomarker of traumatic brain injury (TBI). However, the effect of peripheral injuries such as long bone fracture and skeletal muscle injury on serum NfL levels is unknown. Therefore, the aim of this study was to determine whether serum NfL levels can be used as a biomarker of TBI in the presence of concomitant peripheral injuries.

Methods: Rats were randomly assigned to one of four injury groups: polytrauma (muscle crush + fracture + TBI; n = 11); peripheral injuries (muscle crush + fracture + sham-TBI; n = 12); TBI-only (sham-muscle crush + sham-fracture + TBI; n = 13); and triple-sham (n = 7). At 2-days post-injury, serum levels of NfL were quantified using a Simoa HD-X Analyzer.

Results: Compared to triple-sham rats, serum NfL concentrations were higher in rats with peripheral injuries-only, TBI-only, and polytrauma. When compared to peripheral injury-only rats, serum NfL levels were higher in TBI-only and polytrauma rats. No differences were found between TBI-only and polytrauma rats.

Conclusion: Serum NfL levels did not differ between TBI-only and polytrauma rats, indicating that significant peripheral injuries did not affect the sensitivity and specificity of serum NfL as a biomarker of moderate TBI. However, the finding of elevated serum NfL levels in rats with peripheral injuries in the absence of a TBI suggests that the presence of such injuries may limit the utility of NfL as a biomarker of less severe TBI (eg, concussion).

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血清神经丝光作为伴随外周损伤的创伤性脑损伤的生物标志物。
血清神经丝光(NfL)是一种新兴的创伤性脑损伤(TBI)生物标志物。然而,周围性损伤如长骨骨折和骨骼肌损伤对血清NfL水平的影响尚不清楚。因此,本研究的目的是确定血清NfL水平是否可以作为伴有外周损伤的TBI的生物标志物。方法:将大鼠随机分为4个损伤组:多发伤组(肌肉挤压+骨折+ TBI;n = 11);外周损伤(肌肉挤压+骨折+假性tbi);n = 12);仅TBI(假性肌肉挤压+假性骨折+ TBI;n = 13);和三重sham (n = 7)。损伤后2天,使用Simoa HD-X分析仪定量血清NfL水平。结果:与三次假手术大鼠相比,仅外周损伤、仅tbi和多发损伤大鼠的血清NfL浓度更高。与单纯外周损伤大鼠相比,单纯创伤大鼠和多发损伤大鼠血清NfL水平更高。在单纯创伤大鼠和多发创伤大鼠之间没有发现差异。结论:血清NfL水平在单纯创伤大鼠和多发创伤大鼠之间没有差异,表明明显的外周损伤不影响血清NfL作为中度创伤生物标志物的敏感性和特异性。然而,在没有发生TBI的外周损伤大鼠中发现血清NfL水平升高,这表明这种损伤的存在可能限制了NfL作为轻度TBI(如脑震荡)生物标志物的效用。
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来源期刊
Biomarker Insights
Biomarker Insights MEDICINE, RESEARCH & EXPERIMENTAL-
CiteScore
6.00
自引率
0.00%
发文量
26
审稿时长
8 weeks
期刊介绍: An open access, peer reviewed electronic journal that covers all aspects of biomarker research and clinical applications.
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