Complete sequence of classic F-type plasmid pRK100 shows unique conservation over time and geographic location

IF 1.8 4区 生物学 Q3 GENETICS & HEREDITY Plasmid Pub Date : 2022-01-01 DOI:10.1016/j.plasmid.2022.102618
Marjanca Starčič Erjavec , Karmen Jeseničnik , Lauren P. Elam , Andrej Kastrin , Luka Predojević , Tatyana A. Sysoeva
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Abstract

Plasmids exhibit great diversity of gene content and host ranges and are famous for quick adaptation to the genetic background of the bacterial host cell. In addition to observing ever evolving plasmids, some plasmids have conserved backbones: a stable core composition and arrangement of genes in addition to variable regions. There are a few reports of extremely conserved plasmids. Here we report the complete sequence of pRK100 plasmid – a large, well-characterized conjugative F-like plasmid found in an Escherichia coli strain isolated from a urinary tract infection patient in 1990. The sequence shows that the 142 kb-long pRK100 plasmid is nearly identical to plasmids circulating in distant geographical locations and found in different host E. coli strains between 2007 and 2017. We also performed additional functional characterization of pRK100. Our results showed that pRK100 does not have a strong pathogenicity phenotype in porcine primary bladder epithelial cell culture. Moreover, the conjugation of pRK100 seems to strongly depend on recipient characteristics. These observations and identification of the pRK100 plasmid in different strain genotypes leave the extreme sequence conservation and broad distribution of this plasmid unexplained.

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经典f型质粒pRK100的完整序列在时间和地理位置上具有独特的保守性
质粒具有丰富的基因含量和宿主范围,并以快速适应细菌宿主细胞的遗传背景而闻名。除了观察不断进化的质粒外,一些质粒具有保守的骨干:除了可变区域外,核心组成和基因排列稳定。有一些报道称存在着极其保守的质粒。在这里,我们报告了pRK100质粒的完整序列-一个大的,具有良好特征的共轭f样质粒,发现于1990年从尿路感染患者分离的大肠杆菌菌株。该序列显示,142 kb长的pRK100质粒与2007年至2017年间在不同宿主大肠杆菌菌株中发现的遥远地理位置传播的质粒几乎相同。我们还对pRK100进行了额外的功能表征。结果表明,pRK100在猪原代膀胱上皮细胞培养中不具有强致病性表型。此外,pRK100的结合似乎强烈依赖于受体特征。这些对不同菌株基因型pRK100质粒的观察和鉴定使得该质粒的极端序列保守性和广泛分布无法解释。
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来源期刊
Plasmid
Plasmid 生物-遗传学
CiteScore
4.70
自引率
3.80%
发文量
21
审稿时长
53 days
期刊介绍: Plasmid publishes original research on genetic elements in all kingdoms of life with emphasis on maintenance, transmission and evolution of extrachromosomal elements. Objects of interest include plasmids, bacteriophages, mobile genetic elements, organelle DNA, and genomic and pathogenicity islands.
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