Accurate Neurosurgery for the Establishment of the Electric Kindling Model of Epilepsy in Mice.

IF 2.1 4区 医学 Q2 SURGERY Journal of Investigative Surgery Pub Date : 2022-06-01 Epub Date: 2022-01-30 DOI:10.1080/08941939.2022.2032488
Verónica Custodio, Jorge Acosta, Carmen Rubio, Leonardo Hernández, Javier Brito, Elisa Taddei
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Abstract

This article describes in detail the essential stereotaxic neurosurgery to develop the electric experimental kindling model in mice. To date, available literature describing the methodology of the kindling model is very poor and usually neglects many relevant details about the neurosurgery, such as the manufacture of the electrodes, accurate stereotaxic coordinates of the amygdala nuclei, and the general surgery procedures (e.g., anesthesia, postsurgical recovery, fit survival of the animal's). The electric kindling model produces a progressive development of generalized tonic-clonic seizures, which can be assessed by electroencephalography and behavioral responses. The seizures displayed are produced by a repeated low-intensity electrical stimulation in specific regions of the brain that is achieved through the previous implantation of electrodes. In this study, the aim was to implant the electrodes in basolateral amygdaloid nucleus (BLA). In order to successfully establish the kindling experimental model, neurosurgery to place the electrodes is an essential step to develop the epileptogenic phenomenon. It crucial that the surgery is carried out with exceptional exactitude, because in that way the experimental model represents an accurate and valid tool to study and understand epilepsy and the results obtained can be used to develop further strategies in epilepsy clinical research.

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精确神经外科建立小鼠癫痫电点燃模型。
本文详细介绍了立体定向神经外科建立小鼠电实验点火模型的必要条件。迄今为止,描述点火模型方法的可用文献非常少,并且通常忽略了神经外科的许多相关细节,例如电极的制造,杏仁核的精确立体定位坐标,以及一般手术程序(例如麻醉,术后恢复,动物的适合生存)。电点燃模型产生全身性强直阵挛性发作的渐进发展,这可以通过脑电图和行为反应来评估。所显示的癫痫发作是通过先前植入电极在大脑的特定区域反复进行低强度电刺激而产生的。本研究的目的是将电极植入基底外侧杏仁核(BLA)。为了成功建立引燃实验模型,神经外科放置电极是发展致痫现象的必要步骤。至关重要的是,手术必须非常精确,因为这样,实验模型就代表了研究和了解癫痫的准确和有效的工具,所获得的结果可用于制定癫痫临床研究的进一步策略。
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来源期刊
CiteScore
4.20
自引率
0.00%
发文量
114
审稿时长
6-12 weeks
期刊介绍: Journal of Investigative Surgery publishes peer-reviewed scientific articles for the advancement of surgery, to the ultimate benefit of patient care and rehabilitation. It is the only journal that encompasses the individual and collaborative efforts of scientists in human and veterinary medicine, dentistry, basic and applied sciences, engineering, and law and ethics. The journal is dedicated to the publication of outstanding articles of interest to the surgical research community.
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