Effect of type 2 diabetes mellitus on mandibular bone regeneration and the expression of T helper cell 17/regulat-ory T cell-related factors in mice.

Q3 Medicine 华西口腔医学杂志 Pub Date : 2021-12-01 DOI:10.7518/hxkq.2021.06.004

Ya Nan Wang, Xuan Wu, Ting Ting Jia, Yao Feng, Shi Yue Liu, Xin Xu, Dong Jiao Zhang

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Abstract

Objectives: To observe the effect of type 2 diabetes mellitus (T2DM) on mandibular bone regeneration and the expression of factors related to T helper cell 17 (Th17 cell) and regulatory T cell (Treg cell) in mice.

Methods: Thirty-six 6-week-old C57BL/6J male mice were randomly divided into normal control (NC) and T2DM groups. Fasting blood glucose levels were detected 0 d, 7 d, 14 d, and 28 d after surgery for mandibular defects. Hematoxylin-eosin (HE) staining was used in observing the bone after 7 d, 14 d, and 28 d of the healing process. Immunohistochemical staining was used in observing the expression of alkaline phosphatase (ALP), Runt-related transcription factor 2 (RUNX2), forkhead box protein P3 (Foxp3), retinoic acid related orphan receptor gamma T (RORγt), and protein tyrosine phosphatase non-receptor type 2 (PTPN2) after 7 d, 14 d, and 28 d of healing.

Results: HE staining showed that the area with new bones in the T2DM group was significantly smaller than that in the NC group. Immunohistochemical staining showed that the expression of osteogenesis related proteins ALP and RUNX2 were significantly reduced in the T2DM group. In addition, the number of RORγt positive cells increased, whereas the number of Foxp3 positive cells and the expression PTPN2 decreased significantly in the mandibular bone defect in mice with T2DM.

Conclusions: T2DM significantly inhibit mandibular bone regeneration in mice. Decline in PTPN2 expression and the transition of Treg and Th17 may be the underlying molecular mechanisms.

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2型糖尿病对小鼠下颌骨再生和T辅助细胞17/调节性T细胞相关因子表达的影响。

目的：观察2型糖尿病（T2DM）对小鼠下颌骨再生的影响以及辅助性T细胞17（Th17细胞）和调节性T细胞（Treg细胞）相关因子的表达。方法：36只6周龄C57BL/6J雄性小鼠随机分为正常对照组（NC）和T2DM组。下颌缺损手术后0天、7天、14天和28天检测空腹血糖水平。在愈合过程的第7天、第14天和第28天之后，使用苏木精-伊红（HE）染色来观察骨。免疫组化染色观察愈合7 d、14 d和28 d后碱性磷酸酶（ALP）、Runt相关转录因子2（RUNX2）、叉头盒蛋白P3（Foxp3）、视黄酸相关孤儿受体γT（RORγT）和蛋白酪氨酸磷酸酶非受体2型（PTPN2）的表达。结果：HE染色显示T2DM组新生骨面积明显小于NC组。免疫组织化学染色显示，T2DM组成骨相关蛋白ALP和RUNX2的表达显著降低。此外，在T2DM小鼠下颌骨缺损中，RORγt阳性细胞数量增加，而Foxp3阳性细胞数量和PTPN2表达显著减少。结论：T2DM可显著抑制小鼠下颌骨再生。PTPN2表达的下降以及Treg和Th17的转变可能是潜在的分子机制。

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来源期刊

华西口腔医学杂志 Medicine-Medicine (all)

CiteScore

0.80

自引率

0.00%

发文量

6397

期刊介绍： West China Journal of Stomatology (WCJS, pISSN 1000-1182, eISSN 2618-0456, CN 51-1169/R), published bimonthly, is a peer-reviewed Open Access journal, hosted by Sichuan university and Ministry of Education of the People's Republic of China. WCJS was established in 1983 and indexed in Medline/Pubmed, SCOPUS, EBSCO, Chemical Abstract(CA), CNKI, WANFANG Data, etc.