Single-nucleotide polymorphisms and activities of indoleamine 2,3-dioxygenase isoforms, IDO1 and IDO2, in tuberculosis patients.

IF 2.7 3区生物学 Hereditas Pub Date : 2022-01-19 DOI:10.1186/s41065-022-00219-y

Tingming Cao, Guangming Dai, Hongqian Chu, Chengcheng Kong, Huijuan Duan, Na Tian, Zhaogang Sun

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Abstract

Purpose: To explore the role and effects of the single-nucleotide polymorphisms (SNPs) of the two functionally related indoleamine 2,3-dioxygenase (IDO) isoforms on IDO activity in the Chinese Han ethnic population.

Methods: A total of 151 consecutive patients of Chinese Han ethnicity (99 men and 52 women; average age 51.92 ± 18.26 years) with pulmonary TB admitted to Beijing Chest Hospital between July 2016 and February 2017 were enrolled in the study. The serum levels of tryptophan (Trp) and its metabolites, IDO1 and IDO2 mRNA levels, and the relationship of IDO1 and IDO2 SNPs with the serum Kyn/Trp ratio in TB patients and healthy controls were examined by LC/ESI-MS/MS analysis. Genomic DNA was isolated from whole blood, and the PCR products were sequenced and analyzed.

Results: In Chinese Han participants, only IDO2 had SNPs R248W and Y359X that affected IDO activity, as determined by the serum Kyn/Trp ratio. IDO1 and IDO2 mRNA levels were inversely related in TB patients and healthy controls.

Conclusions: IDO2 SNPs and the opposite expression pattern of IDO1 and IDO2 affected IDO activity in Chinese Han TB patients.

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结核患者吲哚胺2,3-双加氧酶IDO1和IDO2的单核苷酸多态性和活性

目的:探讨两种功能相关的吲哚胺2,3-双加氧酶(IDO)亚型的单核苷酸多态性(snp)在中国汉族人群IDO活性中的作用和影响。方法:共151例汉族患者(男性99例，女性52例;2016年7月至2017年2月在北京胸科医院住院的肺结核患者(平均年龄51.92±18.26岁)纳入研究。采用LC/ESI-MS/MS分析检测TB患者和健康对照组血清色氨酸(Trp)及其代谢物水平、IDO1和IDO2 mRNA水平，以及IDO1和IDO2 snp与血清Kyn/Trp比值的关系。从全血中分离基因组DNA，对PCR产物进行测序和分析。结果:根据血清Kyn/Trp比值，在中国汉族参与者中，只有IDO2具有影响IDO活性的SNPs R248W和Y359X。IDO1和IDO2 mRNA水平在结核病患者和健康对照中呈负相关。结论:IDO2 snp及IDO1和IDO2的相反表达模式影响中国汉族结核患者的IDO活性。

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来源期刊

Hereditas Biochemistry, Genetics and Molecular Biology-Genetics

CiteScore

3.80

自引率

3.70%

发文量

期刊介绍： For almost a century, Hereditas has published original cutting-edge research and reviews. As the Official journal of the Mendelian Society of Lund, the journal welcomes research from across all areas of genetics and genomics. Topics of interest include human and medical genetics, animal and plant genetics, microbial genetics, agriculture and bioinformatics.