Analysis of CYP450 gene expression and function in white-rot fungus, Lenzites gibbosa, treated with Congo red.

IF 1.4 4区生物学 Q4 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Biotechnic & Histochemistry Pub Date : 2022-10-01 Epub Date: 2022-01-24 DOI:10.1080/10520295.2022.2028307

Jian Zhang, YuJie Chi, Jun Zhang

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引用次数: 1

Abstract

We investigated the function of cytochrome P450 (CYP450) genes in degradation of the diazo dye, Congo red, by white-rot fungus Lenzites gibbosa. Hyphae treated with Congo red at different times were sequenced to obtain transcription data. CYP450 genes in transcriptomes were identified using a gene-encoding protein functional search and analyzed in Cluster of Orthologous Genes, Gene Ontology, Kyoto Encyclopedia of Genes and Genomes Databases. Differentially expressed genes (DEGs) in different groups were analyzed using EdgeR. We present the relation between transcription factors (TFs) and CYP450 regulation by analysis of the co-expression network. One hundred sixty CYP450 genes obtained by a functional annotation search were related to the oxido-reduction reaction of secondary metabolism, defense mechanism and aromatic compound degradation. The fastest decolorization and the greatest expression of CYP450 genes, which were related to the decolorization effect, occurred at 0-3 h. Seven CYP450 genes (7522, 6568, 4482, 9118, 10935, 7521 and 10926) were identified. The key TFs that regulate these genes belong to the zinc finger family. CYP450 genes and their products in L. gibbosa participated in degradation of Congo red and stress resistance. We provide a reference value for degradation and decolorization of Congo red.

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刚果红处理后白腐菌CYP450基因表达及功能分析。

我们研究了细胞色素P450 (CYP450)基因在白腐菌Lenzites gibbosa降解重氮染料刚果红中的作用。对不同时间用刚果红处理的菌丝进行测序，获得转录数据。利用基因编码蛋白功能搜索对转录组中CYP450基因进行鉴定，并在同源基因集群、基因本体、京都基因与基因组百科数据库中进行分析。EdgeR分析各组差异表达基因(DEGs)。我们通过分析共表达网络提出了转录因子(TFs)与CYP450调控之间的关系。通过功能注释搜索得到160个CYP450基因，这些基因与次生代谢的氧化还原反应、防御机制和芳香族化合物降解有关。与脱色效果相关的CYP450基因在0-3 h脱色速度最快，表达量最大。共鉴定到7个CYP450基因(7522、6568、4482、9118、10935、7521和10926)。调节这些基因的关键tf属于锌指家族。L. gibbosa CYP450基因及其产物参与了刚果红的降解和抗逆性。为刚果红的降解和脱色提供参考价值。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Biotechnic & Histochemistry 生物-生物工程与应用微生物

CiteScore

3.40

自引率

6.20%

发文量

审稿时长

6-12 weeks

期刊介绍： Biotechnic & Histochemistry (formerly Stain technology) is the official publication of the Biological Stain Commission. The journal has been in continuous publication since 1926. Biotechnic & Histochemistry is an interdisciplinary journal that embraces all aspects of techniques for visualizing biological processes and entities in cells, tissues and organisms; papers that describe experimental work that employs such investigative methods are appropriate for publication as well. Papers concerning topics as diverse as applications of histochemistry, immunohistochemistry, in situ hybridization, cytochemical probes, autoradiography, light and electron microscopy, tissue culture, in vivo and in vitro studies, image analysis, cytogenetics, automation or computerization of investigative procedures and other investigative approaches are appropriate for publication regardless of their length. Letters to the Editor and review articles concerning topics of special and current interest also are welcome.