Messenger RNA profile analysis deciphers new Esrrb responsive genes in prostate cancer cells

IF 2.946 Q3 Biochemistry, Genetics and Molecular Biology BMC Molecular Biology Pub Date : 2015-12-01 DOI:10.1186/s12867-015-0049-1

Yuan Lu, Jilong Li, Jianlin Cheng, Dennis B. Lubahn

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引用次数: 13

Abstract

Orphan nuclear receptor estrogen related receptor β (Esrrb or ERRβ) is well known in stem cells and early embryonic development. However, little is known about its function in cancer.

We investigated the mRNA profile alterations induced by Esrrb expression and its synthetic ligand DY131 in human prostate cancer DU145 cells via RNA-Seq analysis.

We distinguished 67 mRNAs differentially expressed by Esrrb alone. Although DY131 alone did not change any gene, treatment of DY131 in the presence of Esrrb altered 1161 mRNAs. These observations indicated Esrrb had both ligand-independent and ligand-dependent activity. When Esrrb was expressed, DY131 treatment further regulated 15 Esrrb-altered mRNAs. DY131 acted as an antagonist for 11 of 15 mRNAs (wdr52, f13a1, pxdn, spns2, loc100506599, tagln, loc441454, tkel1, sema3f, zcwpw2, sdc2) and as an agonist for 4 of the 15 mRNAs (rarres3, oasl, padi2, ddx60). Gene ontology analyses showed altered genes are related to transcription and translation regulation, cell proliferation and apoptosis regulation, and cellular metabolism.

Our results characterized mRNA profiles in DU145 prostate cancer cells driven by Esrrb expression and Esrrb ligand DY131, and provided multiple markers to characterize Esrrb’s function in Esrrb research.

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信使RNA谱分析破译前列腺癌细胞中新的Esrrb应答基因

孤儿核受体雌激素相关受体β (Esrrb或ERRβ)在干细胞和早期胚胎发育中是众所周知的。然而，人们对它在癌症中的作用知之甚少。我们通过RNA-Seq分析研究了Esrrb表达及其合成配体DY131在人前列腺癌DU145细胞中诱导的mRNA谱变化。我们区分了67个单独由Esrrb差异表达的mrna。虽然单独使用DY131不会改变任何基因，但在Esrrb存在的情况下处理DY131会改变1161个mrna。这些观察结果表明，Esrrb具有不依赖配体和依赖配体的活性。当Esrrb表达时，DY131治疗进一步调节了15种Esrrb改变的mrna。DY131可作为15种mrna中的11种(wdr52、f13a1、pxdn、spns2、loc100506599、tagln、loc441454、tkel1、sema3f、zcwpw2、sdc2)的拮抗剂，并可作为15种mrna中的4种(rarres3、oasl、padi2、ddx60)的激动剂。基因本体分析表明，基因改变与转录和翻译调控、细胞增殖和凋亡调控以及细胞代谢有关。我们的研究结果表征了Esrrb表达和Esrrb配体DY131驱动的DU145前列腺癌细胞的mRNA谱，并提供了多个标记来表征Esrrb在Esrrb研究中的功能。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

BMC Molecular Biology 生物-生化与分子生物学

CiteScore

4.80

自引率

0.00%

发文量

审稿时长

>12 weeks

期刊介绍： BMC Molecular Biology is an open access journal publishing original peer-reviewed research articles in all aspects of DNA and RNA in a cellular context, encompassing investigations of chromatin, replication, recombination, mutation, repair, transcription, translation and RNA processing and function.