MIR4435-2HG, miR-125b-5p, and Sema4D axis affects the aggressiveness of colorectal cancer cells.

IF 2.2 4区生物学 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY Folia histochemica et cytobiologica Pub Date : 2022-01-01 Epub Date: 2022-06-22 DOI:10.5603/FHC.a2022.0018

Ming Yu, Zhengguo Yi, Shenghui Chen, Xiaopeng Chen, Xiaofeng Xie

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引用次数: 3

Abstract

Introduction: The purpose of this study is to elucidate the impact of long non-coding RNA (lncRNA) MIR4435-2HG/microRNA (miR)-125b-5p/ Semaphorins 4D (Sema4D) on colorectal cancer (CRC) cell propagation and migration.

Material and methods: Sema4D expression in 73 pairs of CRC tissues and matched adjacent normal tissues was measured by qRT-PCR and western blot and its association with pathological characteristics of CRC patients was analyzed by chi-square test. Also, the expression of MIR4435-2HG, miR-125b-5p and Sema4D in CRC cell lines was detected by qRT-PCR and Western blot. Knockdown or overexpression of MIR4435-2HG, miR-125b- 5p and Sema4D were separately performed in Caco-2 and LoVo cells, and the cell propagation, migration and invasiveness were detected by cell-counting kit 8, scratch, and transwell assays.

Results: LncRNA MIR4435-2HG and Sema4D were highly expressed, while miR-125b-5p expression was decreased in CRC tissues and cells. Knockdown of MIR4435-2HG/Sema4D or overexpression of miR-125b-5p inhibited CRC cell proliferation and aggressiveness; overexpression of MIR4435-2HG/Sema4D or knockdown of miR-125b-5p prompted the malignant behaviors of cancer cells. MIR4435-2HG and Sema4D competitively bound to miR-125b-5p.

Conclusions: LncRNA MIR4435-2HG targets miR-125b-5p to upregulate Sema4D expression, and thus regulates CRC cell propagation, migration and invasiveness.

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MIR4435-2HG、miR-125b-5p和Sema4D轴影响结直肠癌细胞的侵袭性。

简介:本研究旨在阐明长链非编码RNA (lncRNA) MIR4435-2HG/microRNA (miR)-125b-5p/ Semaphorins 4D (Sema4D)对结直肠癌(CRC)细胞增殖和迁移的影响。材料与方法:采用qRT-PCR和western blot方法检测73对结直肠癌组织及匹配的邻近正常组织中Sema4D的表达，并采用卡方检验分析其与结直肠癌患者病理特征的相关性。采用qRT-PCR和Western blot检测CRC细胞系中MIR4435-2HG、miR-125b-5p和Sema4D的表达。在Caco-2和LoVo细胞中分别下调或过表达MIR4435-2HG、miR-125b- 5p和Sema4D，并通过细胞计数试剂盒8、scratch和transwell检测细胞的增殖、迁移和侵袭性。结果:LncRNA MIR4435-2HG和Sema4D在结直肠癌组织和细胞中高表达，miR-125b-5p表达降低。敲低MIR4435-2HG/Sema4D或过表达miR-125b-5p可抑制结直肠癌细胞的增殖和侵袭性;MIR4435-2HG/Sema4D过表达或miR-125b-5p敲低可促进癌细胞的恶性行为。MIR4435-2HG和Sema4D与miR-125b-5p竞争性结合。结论:LncRNA MIR4435-2HG靶向miR-125b-5p上调Sema4D表达，从而调控结直肠癌细胞的增殖、迁移和侵袭性。

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来源期刊

Folia histochemica et cytobiologica 生物-生化与分子生物学

CiteScore

2.80

自引率

6.70%

发文量

审稿时长

6-12 weeks

期刊介绍： "Folia Histochemica et Cytobiologica" is an international, English-language journal publishing articles in the areas of histochemistry, cytochemistry and cell & tissue biology. "Folia Histochemica et Cytobiologica" was established in 1963 under the title: ‘Folia Histochemica et Cytochemica’ by the Polish Histochemical and Cytochemical Society as a journal devoted to the rapidly developing fields of histochemistry and cytochemistry. In 1984, the profile of the journal was broadened to accommodate papers dealing with cell and tissue biology, and the title was accordingly changed to "Folia Histochemica et Cytobiologica". "Folia Histochemica et Cytobiologica" is published quarterly, one volume a year, by the Polish Histochemical and Cytochemical Society.