Engineering sorghum for higher 4-hydroxybenzoic acid content

IF 3.7 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Metabolic Engineering Communications Pub Date : 2022-12-01 DOI:10.1016/j.mec.2022.e00207
Chien-Yuan Lin , Yang Tian , Kimberly Nelson-Vasilchik , Joel Hague , Ramu Kakumanu , Mi Yeon Lee , Venkataramana R. Pidatala , Jessica Trinh , Christopher M. De Ben , Jutta Dalton , Trent R. Northen , Edward E.K. Baidoo , Blake A. Simmons , John M. Gladden , Corinne D. Scown , Daniel H. Putnam , Albert P. Kausch , Henrik V. Scheller , Aymerick Eudes
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引用次数: 1

Abstract

Engineering bioenergy crops to accumulate coproducts in planta can increase the value of lignocellulosic biomass and enable a sustainable bioeconomy. In this study, we engineered sorghum with a bacterial gene encoding a chorismate pyruvate-lyase (ubiC) to reroute the plastidial pool of chorismate from the shikimate pathway into the valuable compound 4-hydroxybenzoic acid (4-HBA). A gene encoding a feedback-resistant version of 3-deoxy-d-arabino-heptulonate-7-phosphate synthase (aroG) was also introduced in an attempt to increase the carbon flux through the shikimate pathway. At the full maturity and senesced stage, two independent lines that co-express ubiC and aroG produced 1.5 and 1.7 dw% of 4-HBA in biomass, which represents 36- and 40-fold increases compared to the titer measured in wildtype. The two transgenic lines showed no obvious phenotypes, growth defects, nor alteration of cell wall polysaccharide content when cultivated under controlled conditions. In the field, when harvested before grain maturity, transgenic lines contained 0.8 and 1.2 dw% of 4-HBA, which represent economically relevant titers based on recent technoeconomic analysis. Only a slight reduction (11–15%) in biomass yield was observed in transgenics grown under natural environment. This work provides the first metabolic engineering steps toward 4-HBA overproduction in the bioenergy crop sorghum to improve the economics of biorefineries by accumulating a value-added coproduct that can be recovered from biomass and provide an additional revenue stream.

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工程高粱具有较高的4-羟基苯甲酸含量
改造生物能源作物,在植物中积累副产品,可以增加木质纤维素生物质的价值,实现可持续的生物经济。在这项研究中,我们用一种编码choris酸丙酮酸裂解酶(ubiC)的细菌基因改造高粱,将choris酸的质体池从莽草酸途径转变为有价值的化合物4-羟基苯甲酸(4-HBA)。一个编码3-脱氧-d-阿拉伯-庚二酸-7-磷酸合成酶(aroG)的抗反馈基因也被引入,试图通过莽草酸途径增加碳通量。在完全成熟和衰老阶段,两个共同表达ubiC和aroG的独立品系在生物量中产生了1.5和1.7 dw%的4-HBA,与野生型相比,分别增加了36倍和40倍。在控制条件下培养时,两种转基因系均无明显表型、生长缺陷和细胞壁多糖含量变化。在田间,当在籽粒成熟前收获时,转基因品系含有0.8和1.2 dw%的4-HBA,根据最近的技术经济分析,这代表了经济上相关的滴度。在自然环境下生长的转基因作物的生物量产量仅略有下降(11-15%)。这项工作为生物能源作物高粱中4-HBA过量生产提供了第一个代谢工程步骤,通过积累可从生物质中回收的增值副产品来提高生物精炼厂的经济效益,并提供额外的收入来源。
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来源期刊
Metabolic Engineering Communications
Metabolic Engineering Communications Medicine-Endocrinology, Diabetes and Metabolism
CiteScore
13.30
自引率
1.90%
发文量
22
审稿时长
18 weeks
期刊介绍: Metabolic Engineering Communications, a companion title to Metabolic Engineering (MBE), is devoted to publishing original research in the areas of metabolic engineering, synthetic biology, computational biology and systems biology for problems related to metabolism and the engineering of metabolism for the production of fuels, chemicals, and pharmaceuticals. The journal will carry articles on the design, construction, and analysis of biological systems ranging from pathway components to biological complexes and genomes (including genomic, analytical and bioinformatics methods) in suitable host cells to allow them to produce novel compounds of industrial and medical interest. Demonstrations of regulatory designs and synthetic circuits that alter the performance of biochemical pathways and cellular processes will also be presented. Metabolic Engineering Communications complements MBE by publishing articles that are either shorter than those published in the full journal, or which describe key elements of larger metabolic engineering efforts.
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