Liquid chromatography and capillary electrophoresis in glycomic and glycoproteomic analysis.

IF 1.7 4区化学 Q3 CHEMISTRY, MULTIDISCIPLINARY Monatshefte Fur Chemie Pub Date : 2022-01-01 Epub Date: 2022-06-21 DOI:10.1007/s00706-022-02938-4

Katarina Molnarova, Katerina Cokrtova, Alice Tomnikova, Tomas Krizek, Petr Kozlik

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引用次数: 2

Abstract

Glycosylation is one of the most significant and abundant post-translational modifications in cells. Glycomic and glycoproteomic analyses involve the characterization of oligosaccharides (glycans) conjugated to proteins. Glycomic and glycoproteomic analysis is highly challenging because of the large diversity of structures, low abundance, site-specific heterogeneity, and poor ionization efficiency of glycans and glycopeptides in mass spectrometry (MS). MS is a key tool for characterization of glycans and glycopeptides. However, MS alone does not always provide full structural and quantitative information for many reasons, and thus MS is combined with some separation technique. This review focuses on the role of separation techniques used in glycomic and glycoproteomic analyses, liquid chromatography and capillary electrophoresis. The most important separation conditions and results are presented and discussed.

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糖组学和糖蛋白组学分析的液相色谱和毛细管电泳。

糖基化是细胞中最重要和最丰富的翻译后修饰之一。糖组学和糖蛋白组学分析涉及结合到蛋白质上的寡糖(聚糖)的表征。由于质谱(MS)中多糖和糖肽的结构多样性大、丰度低、位点特异性异质性和电离效率差，糖组学和糖蛋白组学分析具有很高的挑战性。质谱是表征多糖和糖肽的重要工具。然而，由于多种原因，质谱本身并不总是能提供完整的结构和定量信息，因此，质谱与一些分离技术相结合。本文综述了分离技术在糖组学和糖蛋白组学分析、液相色谱和毛细管电泳中的作用。介绍并讨论了最重要的分离条件和结果。图形化的简介:

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来源期刊

Monatshefte Fur Chemie 化学-化学综合

CiteScore

3.70

自引率

5.60%

发文量

116

审稿时长

3.3 months

期刊介绍： "Monatshefte für Chemie/Chemical Monthly" was originally conceived as an Austrian journal of chemistry. It has evolved into an international journal covering all branches of chemistry. Featuring the most recent advances in research in analytical chemistry, biochemistry, inorganic, medicinal, organic, physical, structural, and theoretical chemistry, Chemical Monthly publishes refereed original papers and a section entitled "Short Communications". Reviews, symposia in print, and issues devoted to special fields will also be considered.