Impact of Storage Conditions and Mold Types on Aflatoxin B1 Concentration in Corn Residue used as Dairy Feed in Small Holder Dairy Farms, Thailand.

Abstract

The aims of this study were to determine the impact of storage practice and mold types on mold growth and aflatoxin B₁ (AFB₁) concentration in corn residue from local seed corn plants, the main roughage source of dairy farms in the northern region in Thailand. A total of 223 samples from 2 types of corn residue - dried and wet - were collected. Mold contamination was determined by spread plate technique, and aflatoxin B₁ (AFB₁) quantification was performed by a commercial enzyme-linked immunosorbent assay. Multivariate linear models were created to determine factors associated with fungal quantity and AFB₁ concentration. Results showed that the presence of Cladosporium spp. in the samples was associated with a lower risk of AFB1 contamination (P<0.05). In addition, appropriate storage practices, e.g. keeping feeds under a roof and using floor canvas under feed piles, gave lower risk of mold contamination and decreasing AFB₁ contamination.