Investigation of the protective and therapeutic effects of Lactobacillus casei and Saccharomyces cerevisiae in a breast cancer mouse model.

IF 2.7 Q3 MICROBIOLOGY AIMS Microbiology Pub Date : 2022-05-16 eCollection Date: 2022-01-01 DOI:10.3934/microbiol.2022016
Kholoud Baraka, Rania Abozahra, Maged Wasfy Helmy, Nada Salah El Dine El Meniawy, Sarah M Abdelhamid
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Abstract

Introduction: The development of novel strategies for cancer therapy is crucial to improve standard treatment protocols.

Aim: This study aimed to determine the protective and therapeutic effects of heat-killed preparations of Lactobacillus casei and Saccharomyces cerevisiae in a breast cancer mouse model.

Methods: Forty-two female BALB/c mice (7-8 weeks old) were divided into six groups (seven mice per group). Four groups were injected with 107 Ehrlich ascites tumor (EAT) cells suspended in phosphate-buffered saline (PBS) subcutaneously into the left side of the mammary fat pad. Tumor growth was monitored weekly until all animals developed a palpable tumor. The tumor-bearing mice in the experimental groups received heat-killed L. casei or S. cerevisiae three times per week for 35 days. The mice in the control group received PBS. The remaining two groups received heated L. casei or S. cerevisiae and then were injected with EAT cells. After 35 days, all mice were sacrificed to determine the immune response.

Results: Animals that received heated S. cerevisiae exhibited the lowest rate of tumor growth compared with the other groups. TGF-β and IL-4 secretion was increased in all mice, whereas the secretion of INF-γ and IL-10 was decreased in breast tissues. Moreover, at the histopathological level, the volume of viable tumor in the control group was higher than in the treated groups.

Conclusion: Supplementary treatment with S. cerevisiae resulted in the best outcome in the breast cancer model compared with other treated and vaccinated groups.

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研究乳酸杆菌和酿酒酵母菌对乳腺癌小鼠模型的保护和治疗作用。
简介:开发癌症治疗的新策略对于改进标准治疗方案至关重要:目的:本研究旨在确定乳酸杆菌和酿酒酵母菌热杀灭制剂在乳腺癌小鼠模型中的保护和治疗效果:42只雌性BALB/c小鼠(7-8周大)分为6组(每组7只)。四组在左侧乳腺脂肪垫皮下注射 107 个悬浮于磷酸盐缓冲盐水(PBS)中的艾氏腹水瘤(EAT)细胞。每周监测肿瘤生长情况,直到所有动物都长出可触摸到的肿瘤。实验组的肿瘤小鼠每周接受三次热处理杀死的干酪乳杆菌或谷胱甘肽,持续 35 天。对照组的小鼠服用 PBS。其余两组接受加热的干酪乳杆菌或麦角菌,然后注射 EAT 细胞。35 天后,所有小鼠均被处死,以确定免疫反应:结果:与其他组相比,接受加热的麦角菌的动物肿瘤生长率最低。所有小鼠的 TGF-β 和 IL-4 分泌均有所增加,而乳腺组织中 INF-γ 和 IL-10 的分泌则有所减少。此外,在组织病理学水平上,对照组的存活肿瘤体积高于治疗组:结论:与其他治疗组和疫苗接种组相比,用麦角菌辅助治疗乳腺癌模型的效果最好。
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来源期刊
AIMS Microbiology
AIMS Microbiology MICROBIOLOGY-
CiteScore
7.00
自引率
2.10%
发文量
22
审稿时长
8 weeks
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