Characteristics of Virulence Factors and Prevalence of Virulence Markers in Resistant Escherichia coli from Patients with Gut and Urinary Infections in Lafia, Nigeria.

Microbiology insights Pub Date : 2022-06-28 eCollection Date: 2022-01-01 DOI:10.1177/11786361221106993
Olukayode Olugbenga Orole, Salihu Moses Gambo, Victor Stephen Fadayomi
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引用次数: 2

Abstract

The spread and transfer of resistant pathogens is on the increase worldwide and it is presently a cause of concern for health facilities, health organizations and governments. Pathogenicity is a factor dependent on the virulence of the microorganisms. The study aimed at determining the virulence markers and factors in multidrug resistant (MDR) Escherichia coli isolated from patients with urinary tract and gastrointestinal tract infections in Lafia, Nigeria. Collection of urine and stool samples (150 each) from patients was carried out, and bacteria isolated from the samples using the spread plate technique. Antibiotic susceptibility test was determined to identify resistant E. coli isolates after which, virulence factors and genes conferring virulence evaluated. The prevalence of E. coli was 33.3% and 35.3% in urine and stool respectively with 42 of the isolates being MDR. All the isolates showed cell surface hydrophobicity on ammonia sulfate molarity at >1.5, and all possessed capacity to produce hemolysin and pyrogen, though isolate U6 produced the highest amount of hemolysin and the other isolates mostly produced reasonable amount of pyrogen. Isolate U19 from urine sample and isolates S6, S10, S11, and S17 from stool samples all had between 81 and 100 serum resistance survival percentages, while 13 of the isolates had no serum resistance capabilities. Virulence conferring genes present in the isolates include fimH, pap, stb, cs31a, vt2, east1. Most of the resistant isolates have more than one virulence marker that is a means of producing an effective pathogenesis.

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尼日利亚拉菲亚地区肠道和尿路感染患者耐药大肠杆菌毒力因子特征及毒力标记物流行
耐药病原体的传播和转移在世界范围内呈上升趋势,目前已引起卫生机构、卫生组织和各国政府的关注。致病性是一个取决于微生物毒力的因素。该研究旨在确定从尼日利亚拉菲亚尿路和胃肠道感染患者中分离出的耐多药大肠杆菌的毒力标记物和因素。收集患者的尿液和粪便样本(各150份),并使用涂布板技术从样本中分离细菌。采用抗生素药敏试验鉴定耐药大肠杆菌菌株,并对菌株的毒力因子和致病基因进行评价。尿液和粪便中大肠杆菌的检出率分别为33.3%和35.3%,其中42株为耐多药。所有分离株对硫酸铵的量浓度均>1.5时表现出细胞表面疏水性,均具有产生溶血素和热原的能力,但分离株U6产生溶血素最多,其他分离株大多产生适量的热原。尿液分离株U19和粪便分离株S6、S10、S11、S17血清耐药存活率均在81 ~ 100之间,13株无血清耐药能力。在分离株中存在的具有毒力的基因包括fimH, pap, stb, cs31a, vt2, east1。大多数耐药分离株具有一个以上的毒力标记,这是产生有效致病机制的手段。
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