Recombinant human granulocyte colony stimulating factor (rhG-CSF) participates in the progression of implantation via the hsa_circ_0001550-miRNA-mRNA interaction network.

IF 2.1 4区医学 Q2 OBSTETRICS & GYNECOLOGY Human Fertility Pub Date : 2023-12-01 Epub Date: 2022-07-06 DOI:10.1080/14647273.2022.2093137

Meng Lyu, Anchen Su, Lili Zhang, Wenxin Gao, Kun Liu, Feng Yue, Yuanxue Jing, Xiaoling Ma, Lin Liu

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引用次数: 1

Abstract

Inadequate endometrial receptivity is a key factor affecting the successful implantation of embryos. Recombinant human granulocyte colony stimulating factor (rhG-CSF) can increase endometrial thickness and improve the outcomes of assisted reproductive technologies (ARTs). In this preliminary study, the function and possible molecular mechanisms of recombinant human granulocyte colony stimulating factor (rhG-CSF) which affects endometrial receptivity and implantation in human Embryonic Stem Cells (hESCs) were investigated. The cell viability of endometrial stromal cells treated with rhG-CSF 0.5 ng/ml for 24 h was significantly increased. Moreover, the expression of hsa_circ_0001550 was downregulated in endometrial stromal cells treated with rhG-CSF. Furthermore, the hsa_circ_0001550-miRNA-mRNA network was constructed and the downstream target genes (including 4 miRNAs and 117 mRNAs) of hsa_circ_0001550 were mainly involved in the cAMP and calcium signalling pathways, which play important roles in regulating endometrial receptivity and embryo implantation. We conclude that rhG-CSF participates in the regulation of embryo implantation by regulating the hsa_circ_0001550-miRNA-mRNA interaction network.

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重组人粒细胞集落刺激因子（rhG-CSF）通过 hsa_circ_0001550-miRNA-mRNA 相互作用网络参与植入过程。

子宫内膜接受能力不足是影响胚胎成功植入的一个关键因素。重组人粒细胞集落刺激因子（rhG-CSF）可增加子宫内膜厚度，改善辅助生殖技术（ART）的效果。在这项初步研究中，研究人员调查了重组人粒细胞集落刺激因子（rhG-CSF）影响子宫内膜接受性和人类胚胎干细胞（hESCs）植入的功能和可能的分子机制。经0.5 ng/ml的rhG-CSF处理24小时后，子宫内膜基质细胞的细胞活力明显提高。此外，经 rhG-CSF 处理的子宫内膜基质细胞中 hsa_circ_0001550 的表达下调。此外，我们还构建了 hsa_circ_0001550-miRNA-mRNA 网络，发现 hsa_circ_0001550 的下游靶基因（包括 4 个 miRNA 和 117 个 mRNA）主要参与 cAMP 和钙信号通路，而 cAMP 和钙信号通路在调控子宫内膜受孕率和胚胎着床中发挥着重要作用。我们的结论是，rhG-CSF通过调节hsa_circ_0001550-miRNA-mRNA相互作用网络参与胚胎着床的调控。

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来源期刊

Human Fertility OBSTETRICS & GYNECOLOGY-REPRODUCTIVE BIOLOGY

CiteScore

3.30

自引率

5.30%

发文量

期刊介绍： Human Fertility is a leading international, multidisciplinary journal dedicated to furthering research and promoting good practice in the areas of human fertility and infertility. Topics included span the range from molecular medicine to healthcare delivery, and contributions are welcomed from professionals and academics from the spectrum of disciplines concerned with human fertility. It is published on behalf of the British Fertility Society. The journal also provides a forum for the publication of peer-reviewed articles arising out of the activities of the Association of Biomedical Andrologists, the Association of Clinical Embryologists, the Association of Irish Clinical Embryologists, the British Andrology Society, the British Infertility Counselling Association, the Irish Fertility Society and the Royal College of Nursing Fertility Nurses Group. All submissions are welcome. Articles considered include original papers, reviews, policy statements, commentaries, debates, correspondence, and reports of sessions at meetings. The journal also publishes refereed abstracts from the meetings of the constituent organizations.