Allele Specific Polymerase Chain Reaction (AS-PCR) Assay to Detect Knockdown and Acetylcholinesterase Mutations in Anopheles superpictus

Q3 Medicine Turkiye parazitolojii dergisi Pub Date : 2022-11-28 DOI:10.4274/tpd.galenos.2022.20592

Sare İlknur Yavaşoğlu, Fatih Mehmet Şimşek

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Abstract

Objective: The study aims to determine the presence of L1014F, L1014S, L1014C alleles, which are responsible for knockdown resistance and Ace-1 G119S alleles, which are responsible for acetylcholinesterase insensitivity in Anopheles superpictus, the secondary vector of malaria in Turkey.

Methods: In this study, 60 Anopheles superpictus adult females were collected from Aydın, Denizli, and Muğla provinces. Then, allele-specific primers for kdr L1014F, L1014S, and L1014C alleles, and the Ace-1 G119S allele were designed. The presence of these alleles was screened in three Anopheles superpictus populations by allele-specific polymerase chain reaction.

Results: Although L1014S allele frequency was too low in Aydın, Muğla, and Denizli populations, neither kdr L1014F and L1014C nor Ace-1 G119S mutations were found in any population.

Conclusion: In this study, kdr L1014S mutation was detected for the first time in the Aegean Anopheles superpictus populations.

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等位基因特异性聚合酶链反应(AS-PCR)检测超照按蚊乙酰胆碱酯酶敲低和突变

目的:研究土耳其疟疾第二媒介超按蚊中是否存在导致抗敲除的L1014F、L1014S、L1014C等位基因和导致乙酰胆碱酯酶不敏感的Ace-1 G119S等位基因。方法:在Aydın、Denizli和Muğla三省采集超按蚊成虫60只。然后设计kdr L1014F、L1014S、L1014C等位基因和Ace-1 G119S等位基因的等位基因特异性引物。采用等位基因特异性聚合酶链反应对3个超按蚊种群进行了筛选。结果:虽然L1014S等位基因频率在Aydın、Muğla和Denizli人群中过低，但在所有人群中均未发现kdr L1014F、L1014C和Ace-1 G119S突变。结论:本研究首次在爱琴海超按蚊种群中检测到kdr L1014S突变。

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