MicroRNA-145-5p modulates Krüppel-like factor 5 and inhibits cell proliferation, migration, and invasion in nasopharyngeal carcinoma.

IF 2.7 3区生物学 Q4 CELL BIOLOGY BMC Molecular and Cell Biology Pub Date : 2022-07-14 DOI:10.1186/s12860-022-00430-9

Chien-Han Yuan, Wei-Chi Hsu, A-Mei Huang, Ben-Chih Yuan, I-Hung Chen, Chia-An Hsu, Rong-Feng Chen, Yih-Min Chu, Hui-Hui Lin, Hung-Lung Ke

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引用次数: 3

Abstract

Background: In several human cancers, Krüppel-like factor 5 (KLF5), a zinc finger transcription factor, can contribute to both tumor progression or suppression; however, the precise role of KLF5 in nasopharyngeal carcinoma (NPC) remains poorly understood. In this study, the association between KLF5 and microRNA-145-5p (miR-145-5p) in NPC cells was elucidated.

Results: Our results showed that KLF5 expression was up-regulated in NPC group compared to normal group. We found that KLF5 exhibited an oncogenic role in NPC cells. The upregulation of miR-145-5p inhibited the proliferation, migration, and invasion of NPC cells. It was observed that miR-145-5p could down-regulate the mRNA and protein expression of KLF5 in NPC cell lines. Additionally, the activity of focal adhesion kinase (FAK), a migration marker, was regulated by miR-145-5p and KLF5 in NPC cells.

Conclusions: The results of this study indicated that miR-145-5p could repress the proliferation, migration, and invasion of NPC cells via KLF5/FAK regulation, and could be a potential therapeutic target for patients with NPC.

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MicroRNA-145-5p调节kr ppel样因子5并抑制鼻咽癌细胞增殖、迁移和侵袭。

背景:在几种人类癌症中，kr ppel样因子5 (KLF5)，一种锌指转录因子，可以促进肿瘤进展或抑制;然而，KLF5在鼻咽癌(NPC)中的确切作用仍然知之甚少。本研究阐明了鼻咽癌细胞中KLF5与microRNA-145-5p (miR-145-5p)之间的关联。结果:我们的研究结果显示，与正常组相比，鼻咽癌组KLF5表达上调。我们发现KLF5在鼻咽癌细胞中表现出致癌作用。miR-145-5p的上调可抑制鼻咽癌细胞的增殖、迁移和侵袭。我们观察到miR-145-5p可以下调鼻咽癌细胞系中KLF5 mRNA和蛋白的表达。此外，在鼻咽癌细胞中，局灶黏附激酶(FAK)的活性受miR-145-5p和KLF5的调控。FAK是一种迁移标志物。结论:本研究结果表明miR-145-5p可通过调控KLF5/FAK抑制鼻咽癌细胞的增殖、迁移和侵袭，可能成为鼻咽癌患者的潜在治疗靶点。

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