Colistin resistance and heteroresistance in Klebsiella pneumoniae & Escherichia coli clinical isolates from intensive care units.

IF 0.5 4区医学 Q4 MICROBIOLOGY Epidemiologie Mikrobiologie Imunologie Pub Date : 2022-01-01

M A Meheissen, S M Hendawy, F S Shabaan, A M Elmenshawy, R A Harfoush

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Abstract

Aim: Colistin resistance poses a serious clinical problem. This study aims to investigate the presence of plasmid-mediated resistance and heteroresistance among Escherichia coli (E. coli) and Klebsiella pneumoniae (K. pneumoniae) clinical isolates at intensive care units of Main Alexandria University Hospital.

Material and methods: Seventy colistin-resistant and 30 colistin-susceptible K. pneumoniae and E. coli isolates constituted the material of the study. The rapid polymyxin NP (Nordmann Poirel) test was performed. Heteroresistance in colistin-susceptible isolates was investigated by population analysis profile. mcr-1 and mcr-2 genes were amplified by polymerase chain reaction (PCR).

Results: The rapid polymyxin NP test showed 100% concordance with the results of BMD (broth microdilution method). Heteroresistance to colistin was detected in 23.3% of colistin-susceptible K. pneumoniae isolates. All colistin-resistant isolates were negative for mcr-1 and mcr-2 genes.

Conclusions: The rapid polymyxin NP test is a reliable screening tool for colistin resistance, but not for heteroresistance. Other colistin resistance mechanisms should be investigated.

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重症监护病房肺炎克雷伯菌和大肠杆菌临床分离株的粘菌素耐药性和异源耐药性。

目的:粘菌素耐药是严重的临床问题。本研究旨在调查亚历山大大学附属医院重症监护病房大肠埃希菌(E. coli)和肺炎克雷伯菌(K. pneumoniae)临床分离株中质粒介导的耐药性和异耐药性的存在。材料和方法:70株耐粘菌素和30株敏感粘菌素肺炎克雷伯菌和大肠杆菌构成本研究的材料。进行快速多粘菌素NP (Nordmann Poirel)试验。用群体分析方法研究了粘菌素敏感株的异耐药情况。采用聚合酶链反应(PCR)扩增mcr-1和mcr-2基因。结果:多粘菌素快速NP试验结果与BMD(肉汤微量稀释法)结果吻合100%。23.3%的肺炎克雷伯菌对粘菌素敏感。所有耐粘菌素菌株均为mcr-1和mcr-2基因阴性。结论:快速多粘菌素NP试验是筛选粘菌素耐药的可靠工具，但不能用于筛选异源耐药。其他的粘菌素耐药机制也有待研究。

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来源期刊

Epidemiologie Mikrobiologie Imunologie Medicine-Immunology and Allergy

CiteScore

0.90

自引率

0.00%

发文量

期刊介绍： The journal publishes original papers, information from practice, reviews on epidemiological and microbiological subjects. Sufficient space is devoted to diagnostic methods from medical microbiology, parasitology, immunology, and to general aspects and discussions pertaining to preventive medicine. It also brings translations and book reviews useful for medical doctors and research workers and professionals in public health.

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