Long non-coding RNA LBX2-AS1 predicts poor survival of colon cancer patients and promotes its progression via regulating miR-627-5p/RAC1/PI3K/AKT pathway.

IF 4.3 3区生物学 Human Cell Pub Date : 2022-09-01 Epub Date: 2022-07-11 DOI:10.1007/s13577-022-00745-x

Jing Fang, Junyuan Yang, Hui Chen, Wen Sun, Lingyun Xiang, Jueping Feng

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引用次数: 3

Abstract

Colon cancer is one of the most prevalent malignant tumors across the world. Increasing studies have demonstrated that long non-coding RNAs (lncRNAs) take part in colon cancer development. Our study intends to explore the expression characteristics of LBX2-AS1, a novel lncRNA, in colon cancer and its underlying mechanisms. The results illustrated that LBX2-AS1 level was substantially increased in colon cancer tissues and was obviously correlated with the tumor volume and early distant metastasis of patients. Besides, overexpression of LBX2-AS1 remarkably boosted growth, proliferation, and metastasis and restrained apoptosis in colon cancer cells, whereas LBX2-AS1 knockdown produced the opposite effect. On the other hand, miR-627-5p, down-regulated in colon cancer tissues, was negatively associated with LBX2-AS1 expression. Functional experiments showed that miR-627-5p suppressed colon cancer growth. Mechanistically, LBX2-AS1, as an endogenous competitive RNA, targeted miR-627-5p and restrained its expression, while miR-627-5p targeted and negatively regulated the RAC1/PI3K/AKT axis. Collectively, this study has revealed that LBX2-AS1 is a poor prognostic factor of colon cancer and can regulate colon cancer progression by regulating the miR-627-5p/RAC1/PI3K/AKT pathway.

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长链非编码RNA LBX2-AS1通过调节miR-627-5p/RAC1/PI3K/AKT通路预测结肠癌患者的不良生存，促进其进展。

结肠癌是世界上最常见的恶性肿瘤之一。越来越多的研究表明，长链非编码rna (lncRNAs)参与结肠癌的发展。本研究旨在探讨新型lncRNA LBX2-AS1在结肠癌中的表达特征及其机制。结果表明，LBX2-AS1水平在结肠癌组织中显著升高，且与肿瘤体积及患者早期远处转移有明显相关性。此外，过表达LBX2-AS1可显著促进结肠癌细胞的生长、增殖和转移，抑制凋亡，而低表达LBX2-AS1则具有相反的作用。另一方面，结肠癌组织中下调的miR-627-5p与LBX2-AS1表达呈负相关。功能实验表明，miR-627-5p抑制结肠癌的生长。机制上，LBX2-AS1作为内源性竞争性RNA靶向miR-627-5p并抑制其表达，而miR-627-5p靶向并负调控RAC1/PI3K/AKT轴。综上所述，本研究揭示了LBX2-AS1是结肠癌的不良预后因子，可通过调控miR-627-5p/RAC1/PI3K/AKT通路调控结肠癌的进展。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Human Cell 生物-细胞生物学

CiteScore

6.60

自引率

2.30%

发文量

176

期刊介绍： Human Cell is the official English-language journal of the Japan Human Cell Society. The journal serves as a forum for international research on all aspects of the human cell, encompassing not only cell biology but also pathology, cytology, and oncology, including clinical oncology. Embryonic stem cells derived from animals, regenerative medicine using animal cells, and experimental animal models with implications for human diseases are covered as well. Submissions in any of the following categories will be considered: Research Articles, Cell Lines, Rapid Communications, Reviews, and Letters to the Editor. A brief clinical case report focusing on cellular responses to pathological insults in human studies may also be submitted as a Letter to the Editor in a concise and short format. Not only basic scientists but also gynecologists, oncologists, and other clinical scientists are welcome to submit work expressing new ideas or research using human cells.